Light-induced c-Fos expression in the mouse suprachiasmatic nucleus: Immunoelectron microscopy reveals co-localization in multiple cell types
Light-induced c-Fos expression in the mouse suprachiasmatic nucleus: Immunoelectron microscopy reveals co-localization in multiple cell types
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DOI：
10.1111/j.1460-9568.1997.tb00762.x
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发表时间：
1997-09-01
影响因子：
3.4
通讯作者：
Schwartz, WJ
中科院分区：
文献类型：
--
作者：
Castel, M;Belenky, M;Schwartz, WJ
虽然光是已知的调节水平的c-fos基因表达的视交叉上核（SCN），一个内源性的昼夜节律钟的网站，鲜为人知的是光激活细胞的身份。我们使用光镜免疫细胞化学和免疫电镜检测c-Fos蛋白在SCN的Sabra小鼠暴露于短暂的夜间光脉冲在zeitgeber时间15-16。光脉冲的刺激，饱和的昼夜运动节律的相移响应揭示了上限的光诱导的c-Fos细胞的数量在约五分之一的估计总SCN细胞群。这个功能定义的集合在形态和表型上是异质的。约24%的c-Fos阳性细胞可标记为血管活性肠肽，13%的加压素-神经垂体素，7%的胶质细胞酸性蛋白。其余56%的c-Fos阳性细胞的表型大多未知，虽然许多推定的中间神经元，其中一些是免疫反应性的一氧化氮合酶。
Although light is known to regulate the level of c-fos gene expression in the suprachiasmatic nucleus (SCN), the site of an endogenous circadian clock, little is known about the identities of the photically activated cells. We used light-microscopic immunocytochemistry and immunoelectron microscopy to detect c-Fos protein in the SCN of Sabra mice exposed to brief nocturnal light pulses at zeitgeber time 15-16. Stimulation with light pulses that saturated the phase-shifting response of the circadian locomotor rhythm revealed an upper limit to the number of photo-inducible c-Fos cells at about one-fifth of the estimated total SCN cell population. This functionally defined set was morphologically and phenotypically heterogeneous. About 24% could be labelled for vasoactive intestinal polypeptide, 13% for vasopressin-neurophysin, and 7% for glial fibrillary acidic protein, The remaining 56% of c-Fos-positive cells were largely of unknown phenotype, although many were presumptive interneurons, some of which were immunoreactive for nitric oxide synthase.