Time Course of Cold Cataract Development in Anesthetized Mice
Time Course of Cold Cataract Development in Anesthetized Mice
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DOI：
10.3109/02713683.2010.542868
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发表时间：
2011-03-01
影响因子：
2
通讯作者：
Gonzalez, Francisco
中科院分区：
文献类型：
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作者：
Bermudez, Maria A.;Vicente, Ana F.;Gonzalez, Francisco
研究方法：我们麻醉5组10只小鼠（12周龄）与400毫克/公斤的水合氯醛腹腔注射，并将它们暴露于0，7，15，23，和37度A摄氏度为1小时。白内障的发展进行了评估，并在10，20，30，45，和60分钟后，曝光开始分级为无白内障，轻度，中度，或重度。为了定量的目的，将0 ~ 3的值分配给每个白内障等级，并计算每个组和时间点的中值（白内障指数，CI）。我们发现，37度A度C组的4只小鼠，23度A度C组的9只小鼠，以及15度、7度和0度A度C组的所有动物都患上了白内障。白内障在暴露于0度A ° C后10分钟开始，在暴露于7、15和23度A ° C后20分钟开始。随着温度的降低，发育速度和CI显著增加。当在15、23和37度A度C组中48小时后重复该程序时，观察到类似的结果。在所有情况下，白内障是可逆的。结论：我们的研究结果表明，冷白内障的发展是温度依赖性的，白内障的形成开始暴露于低温后10和20分钟之间。这一发现与需要透明眼介质的实验环境相关。
Methods: We anesthetized five groups of 10 mice (12 weeks old) with 400 mg/Kg intraperitoneal injections of chloral hydrate and exposed them to 0, 7, 15, 23, and 37 degrees A degrees C for 1 hr. Cataract development was assessed and graded as no cataract, mild, medium, or severe at 10, 20, 30, 45, and 60 min after the exposure started. For quantification purposes, a value from 0 to 3 was assigned to each cataract grade, and the median value was calculated for each group and time point (cataract index, CI).Results: The CI for each temperature fitted a negative exponential equation. We found that four mice of the 37 degrees A degrees C group, nine of the 23 degrees A degrees C group, and all animals of the 15, 7, and 0 degrees A degrees C groups developed cataract. The cataract started at 10 min after exposure to 0 degrees A degrees C and at 20 min when exposed to 7, 15, and 23 degrees A degrees C. The speed of development and CI significantly increased with lower temperatures. Similar results were observed when the procedure was repeated 48 hr later in the 15, 23, and 37 degrees A degrees C groups. In all instances the cataract was reversible.Conclusion: Our findings show that cold cataract development is temperature dependent and that cataract formation starts between 10 and 20 min after exposure to low temperature. This finding is relevant for those experimental settings in which clear ocular media are required.