Isolation and properties of bacteriolytic enzyme-producing cocci from the human mouth.
Isolation and properties of bacteriolytic enzyme-producing cocci from the human mouth.
复制标题
人口腔中产溶菌酶球菌的分离和特性。
作者：
T. Kanamoto;H. Eifuku;M. Inoue
从口腔的不同部位分离出101种产生溶菌酶的微生物。全部为非溶血性、革兰氏阳性和链形成球菌。91株菌株与缺陷链球菌和沙门氏菌的参考菌株相似。邻生体依赖于吡哆醛生长并产生发色团。Rapid ID 32 STREP系统将这些分离株鉴定为S。defectivus、S.邻生或麻疹双生子。其余10个溶菌菌株不依赖吡哆醛，8个属于S。中间体一些不依赖吡哆醛的S.包括ATCC 27335 T在内的中间型肠球菌参考菌株和所有测试的D组肠球菌菌株也是溶菌的。因此，溶菌酶的产生是营养变异链球菌所共有的，而不是链球菌所独有的。defectives和S.邻接营养变异菌株通常具有芳基酰胺酶，但不具有碱性磷酸酶。色葡萄defectivus菌株产生α-和β-半乳糖苷酶（生物型1），而S.邻接菌株通常产生N-乙酰-β-氨基葡萄糖苷酶，并且一些具有β-葡萄糖醛酸酶，但其它不具有（生物型2和3）。梭麻疹菌株没有检测到这些糖苷酶（生物型4）的活性，但产生发色团和精氨酸二氢水解酶，表现出Gemella物种的非典型生理特征。这表明在营养变异链球菌中可能存在另外的表型组或新的菌种。
One-hundred-and-one bacteriolytic enzyme producing organisms were isolated from various sites of the mouth. All were non-hemolytic, Gram-positive, and chain-forming cocci. Ninety-one strains, like the reference strains of Streptococcus defectivus and S. adjacens, were dependent on pyridoxal for growth and produced a chromophore. The Rapid ID32 STREP system speciated these isolates as S. defectivus, S. adjacens or Gemella morbillorum. The remaining 10 bacteriolytic isolates were pyridoxal-independent and 8 belonged to S. intermediate. Some pyridoxal-independent S. intermedius reference strains including ATCC27335T and all group D Enterococcus strains tested were also bacteriolytic. Thus, bacteriolytic enzyme production is common to nutritionally variant streptococci but not unique to S. defectives and S. adjacens. The nutritionally variant strains generally had arylamidases but not alkaline phosphatase. The S. defectivus strains produced alpha-and beta-galactosidases (biotype 1) whereas the S. adjacens strains generally produced N-acetyl-beta-glucosaminidase and some had beta-glucuronidase but others did not (biotypes 2 and 3). The C. morbillorum strains had no detectable activity of these glycosidases (biotype 4) but produced a chromophore and an arginine dihydrolase, exhibiting a physiological profile atypical of the Gemella species. This indicates the possible presence of an additional phenotypic group or a new species among the nutritionally variant streptococci.
营养变异链球菌的免疫化学研究。
DOI：
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发表时间：
1984
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
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作者：
vandeRijn,I;George,M
通讯作者：
George,M