Bioremediation of the tobacco waste-contaminated soil by Pseudomonas sp. HF-1: nicotine degradation and microbial community analysis
Bioremediation of the tobacco waste-contaminated soil by Pseudomonas sp. HF-1: nicotine degradation and microbial community analysis
复制标题
假单胞菌对烟草废物污染土壤的生物修复。
DOI：
10.1007/s00253-012-4433-1
复制
发表时间：
2012-09
影响因子：
5
通讯作者：
Zhenmei Lu
中科院分区：
文献类型：
--
作者：
Yanlai Yao;Haixia Wang;Hang Min;Zhenmei Lu
高效尼古丁降解细菌假单胞菌。将 HF-1 添加到烟草废物污染的土壤中以降解尼古丁并评估生物修复的效果。与不添加（NA）系统相比，添加菌株HF-1（TA）的处理表现出更强的污染处理能力以及更高的pH值和水分含量稳定性，特别是在含有大量烟草废物的组中。变性梯度凝胶电泳（DGGE）曲线显示，NA 处理中香农-维纳指数随着废物的增加而降低，而 TA 组中香农-维纳指数逐渐增加。 DGGE条带序列的比较表明，两种处理之间的优势微生物种类存在差异，这表明菌株HF-1可以在土壤中持续存在并提高烟草废物处理的效率。实时荧光定量PCR（RT-qPCR）结果也表明TA系统中存在菌株HF-1，并且生长量较高。总之，尼古丁降解菌株HF-1在烟草废弃物污染土壤的生物修复中发挥主导作用，并影响微生物群落的动态和结构。
The highly effective nicotine-degrading bacterium Pseudomonas sp. HF-1 was augmented into the tobacco waste-contaminated soil to degrade nicotine and evaluate the effect of the bioremediation. Comparing with non-adding (NA) systems, the treatments with addition of strain HF-1 (TA) exhibited considerably stronger pollution disposal abilities and higher stability of pH value and moisture content, especially in groups containing a large quantity of tobacco waste. The denaturing gradient gel electrophoresis (DGGE) profiles showed that the Shannon–Wiener index decreased with increasing wastes in the NA treatments, while a gradual increase was found in the TA groups. A comparison of sequences from DGGE bands demonstrated that there were differences in the dominant microbial species between the two treatments, suggesting that strain HF-1 could persist in the soil and enhance the efficiency of tobacco waste disposal. The results of real-time fluorescence quantitative PCR (RT-qPCR) also indicated that strain HF-1 existed in the TA systems and grew with relative high quantities. In conclusion, the nicotine-degrading strain HF-1 played a leading role in the bioremediation of the tobacco waste-contaminated soil and influenced the dynamics and structure of the microbial community.
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DOI：
10.1111/j.1651-2227.1944.tb10559.x
发表时间：
1944-12
期刊：
Acta Pædiatrica
影响因子：
--
作者：
James A. Gott
通讯作者：
James A. Gott
DOI：
10.1111/j.1600-0404.1997.tb00594.x
发表时间：
1939-09
期刊：
Music Educators Journal
影响因子：
--
作者：
Laurajane Smith
通讯作者：
Laurajane Smith
影响因子：
3.1
作者：
Jun Wang;Dingqiang Lu;Yao Liang;Hui Zhao;Min Luo;Xiuquan Ling;P. Ouyang
通讯作者：
Jun Wang;Dingqiang Lu;Yao Liang;Hui Zhao;Min Luo;Xiuquan Ling;P. Ouyang
DOI：
--
发表时间：
--
期刊：
--
影响因子：
--
作者：
K. Livak;Thomas D. Schmittgen
通讯作者：
K. Livak;Thomas D. Schmittgen
影响因子：
5.6
作者：
S. Schenk;A. Hoelz;Beate Krauβ;K. Decker
通讯作者：
S. Schenk;A. Hoelz;Beate Krauβ;K. Decker