Propofol activates vanilloid receptor channels expressed in human embryonic kidney 293 cells
Propofol activates vanilloid receptor channels expressed in human embryonic kidney 293 cells
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DOI：
10.1016/s0304-3940(01)02185-1
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发表时间：
2001-10-12
影响因子：
2.5
通讯作者：
Kohama, K
中科院分区：
文献类型：
--
作者：
Tsutsumi, S;Tomioka, A;Kohama, K
丙泊酚（2，6-二异丙基苯酚）是一种静脉麻醉剂，在结构上与任何其他静脉麻醉剂无关。应用钙离子成像技术研究了异丙酚对人胚肾（HEK）293细胞表达的大鼠香草酸受体的影响。异丙酚引起浓度依赖性增加[Ca 2 +]，在HEK 293细胞与受体。这些反应被抑制细胞外钙离子。在HEK 293细胞中，异丙酚引起的[Ca ~（2+）]_i增加与受体部分抑制辣椒碱的竞争性拮抗剂辣椒平。我们得出结论，异丙酚作为受体的激动剂。(C)2001爱思唯尔科学爱尔兰有限公司保留所有权利。
Propofol (2,6-diisopropylphenol) is an intravenous anesthetic agent structurally unrelated to any other intravenous anesthetics. We examined the effect of propofol on a rat vanilloid receptor that was expressed in the human embryonic kidney (HEK) 293 cells by using calcium imaging method. Propofol caused a concentration-dependent increase in [Ca2+], in the HEK293 cells with the receptor. These responses were inhibited by removing extracellular calcium ions. The propofol-evoked increase in [Ca2+], in the HEK293 cells with the receptor was partially inhibited by capsazepine, a competitive antagonist of capsaicin. We conclude that propofol acts as an agonist for the receptor. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.