The gut bacterial microbiome of the Threeridge mussel, Amblema plicata , varies between rivers but shows a consistent core community
The gut bacterial microbiome of the Threeridge mussel, Amblema plicata , varies between rivers but shows a consistent core community
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三脊贻贝 (Amblema plicata) 的肠道细菌微生物组在不同河流中存在差异，但显示出一致的核心群落
DOI：
10.1111/fwb.13905
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发表时间：
2022
影响因子：
2.7
通讯作者：
Jackson, Colin R.
中科院分区：
文献类型：
--
作者：
Lawson, Lauren A.;Atkinson, Carla L.;Jackson, Colin R.
淡水贻贝对营养循环和生态系统健康很重要，因为它们过滤周围的水。这种滤食使这些双壳类对环境条件特别敏感。肠道微生物群落（微生物组）已被认为对宿主生物和生态系统健康都很重要;然而，淡水贻贝微生物组是如何组织和影响的尚不清楚。在这项研究中，在美国东南部的两个流域，五条河流和九个当地地点比较了Threeridge贻贝，Amblema plicata的肠道细菌微生物组。贻贝肠道组织被解剖，提取DNA，和微生物组的特点是高通量测序的V4区的16 S核糖体RNA基因。浮游菌，厚壁菌门，蓝细菌是最常见的细菌门内的所有采样A. plicata的肠道。然而，这些主要的细菌门的相对丰度不同的贻贝样品从不同的河流和流域，作为特定的细菌操作分类单位（OTU）的相对丰度。尽管存在这些差异，但在所有贻贝中鉴定出核心微生物组，其中8个OTU在所有位点都是褶皱曲霉微生物组的一致成员，最丰富的OTU被鉴定为浮游菌科的成员。研究地点之间的地理距离与肠道微生物组结构的相似性无关，而肠道微生物组结构的相似性与研究地点的物理化学更相关。总体而言，这些结果表明，虽然物理化学条件会影响Threeridge贻贝肠道微生物组中瞬时细菌的组成，但核心微生物组基本不受影响，无论河流系统如何，一部分褶皱曲霉菌群都会被保留下来。短暂的细菌在肠道中停留多久，以及这些短暂的微生物在多大程度上帮助宿主功能仍然是未知的。核心微生物群被发现有助于动物宿主的多种功能，而在淡水贻贝中，这种核心微生物群可能有助于营养加工和循环。因此，在研究淡水无脊椎动物微生物组的结构时，重要的是要同时研究瞬时微生物和核心微生物。
Freshwater mussels are important for nutrient cycling and ecosystem health as they filter feed on their surrounding water. This filter feeding makes these bivalves especially sensitive to conditions in their environment. Gut microbial communities (microbiomes) have been recognised as important to both host organism and ecosystem health; however, how freshwater mussel microbiomes are organised and influenced is unclear.In this study, the gut bacterial microbiome of Threeridge mussel,Amblema plicata, was compared across two river basins, five rivers, and nine local sites in the south‐eastern U.S.A. Mussel gut tissue was dissected, DNA extracted, and the microbiome characterised by high throughput sequencing of the V4 region of the 16S ribosomal RNA gene.Planctomycetes, Firmicutes, and Cyanobacteria were the most common bacterial phyla within the guts of all sampledA.plicata. However, the relative abundances of these major bacterial phyla differed between mussels sampled from different rivers and river basins, as did the relative abundance of specific bacterial operational taxonomic units (OTUs). Despite these differences, a core microbiome was identified across all mussels, with eight OTUs being consistent members of theA.plicatamicrobiome at all sites, the most abundant OTU identifying as a member of the family Planctomycetaceae. Geographic distance between sites was not correlated with similarity in the structure of the gut microbiome, which was more related to site physicochemistry.Overall, these results suggest that while physicochemical conditions affect the composition of transient bacteria in the Threeridge mussel gut microbiome, the core microbiome is largely unaffected, and a portion of theA.plicatamicrobiome is retained regardless of the river system.How long transient bacteria remain in the gut, and to what extent these transient microbes aid in host function is still unknown. Core microbiota have been found to aid in multiple functions within animal hosts, and within freshwater mussels this core microbiome may aid in nutrient processing and cycling. Therefore, it is important to look at both transient and core microbes when studying the structure of freshwater invertebrate microbiomes.