Modeling innate immunity in murine skin: utilization of subcutaneous osmotic pumps for inflammatory and fibrotic skin disease.
Modeling innate immunity in murine skin: utilization of subcutaneous osmotic pumps for inflammatory and fibrotic skin disease.
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模拟小鼠皮肤的先天免疫：利用皮下渗透泵治疗炎症和纤维化皮肤病。
DOI：
10.1007/978-1-60761-720-4_15
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Lafyatis,Robert
中科院分区：
文献类型：
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作者：
Dimarzio,Michael;Farina,Giuseppina;Lafyatis,Robert
目前的系统性硬化症模型在模拟人类疾病方面有许多局限性。我们最近描述了一种基于poly（I：C）对皮肤的慢性刺激的新模型，poly（I：C）是一种已知刺激先天免疫反应的双链RNA形式。该模型显示炎症和细胞外基质沉积，以及与纤维化和血管损伤相关的基因表达上调。我们在这里详细介绍了这种模型的方法，利用渗透泵提供先天性免疫刺激皮下组织。皮下渗透泵为研究皮肤中的先天免疫以及其他配体对皮肤炎症和纤维化的影响提供了灵活的系统。
Current models for systemic sclerosis have many limitations for mimicking human disease. We have recently described a novel model based on chronic stimulation of skin by poly(I:C), a form of double-stranded RNA known to stimulate innate immune responses. This model shows inflammation and extracellular matrix deposition, and upregulated expression of genes associated with fibrosis and vascular injury. We detail here the methodology for this model, utilizing osmotic pumps to deliver innate immune stimuli to subcutaneous tissue. Subcutaneous osmotic pumps provide a flexible system for studying innate immunity in the skin as well as the affects of other ligands on skin inflammation and fibrosis.