Global analysis of lysine acetylation in strawberry leaves.
Global analysis of lysine acetylation in strawberry leaves.
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DOI：
10.3389/fpls.2015.00739
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发表时间：
2015
影响因子：
5.6
通讯作者：
Cheng Z
中科院分区：
文献类型：
--
作者：
Fang X;Chen W;Zhao Y;Ruan S;Zhang H;Yan C;Jin L;Cao L;Zhu J;Ma H;Cheng Z
蛋白质赖氨酸乙酰化是一种可逆的动态翻译后修饰。在原核生物和真核生物中，它在调节多种细胞过程，包括染色质动力学、代谢途径和转录中起重要作用。虽然对植物中赖氨酸乙酰化组的研究已有报道，但目前的研究通量还不够高，阻碍了对赖氨酸乙酰化在植物生理和病理中的深入了解。本研究利用抗乙酰赖氨酸富集技术和高灵敏度质谱技术，应用整合蛋白质组学方法对草莓赖氨酸乙酰组进行了全面研究。总的来说，我们在684个蛋白质中鉴定了1392个乙酰化位点，代表了迄今为止植物中最大的乙酰组数据集。为了揭示草莓中赖氨酸乙酰化的功能影响，进行了深入的生物信息学分析。这些结果大大扩展了我们目前对植物乙酰组的理解，并证明赖氨酸乙酰化参与多种细胞代谢和细胞过程。更有趣的是，近50%的乙酰化蛋白质在这项工作中被确定定位在叶绿体和赖氨酸乙酰化在光合作用中的重要作用也被揭示。综上所述，本研究不仅建立了迄今为止植物中最广泛的赖氨酸乙酰化组，而且系统地表明了赖氨酸乙酰化在植物中的重要和独特的作用。
Protein lysine acetylation is a reversible and dynamic post-translational modification. It plays an important role in regulating diverse cellular processes including chromatin dynamic, metabolic pathways, and transcription in both prokaryotes and eukaryotes. Although studies of lysine acetylome in plants have been reported, the throughput was not high enough, hindering the deep understanding of lysine acetylation in plant physiology and pathology. In this study, taking advantages of anti-acetyllysine-based enrichment and high-sensitive-mass spectrometer, we applied an integrated proteomic approach to comprehensively investigate lysine acetylome in strawberry. In total, we identified 1392 acetylation sites in 684 proteins, representing the largest dataset of acetylome in plants to date. To reveal the functional impacts of lysine acetylation in strawberry, intensive bioinformatic analysis was performed. The results significantly expanded our current understanding of plant acetylome and demonstrated that lysine acetylation is involved in multiple cellular metabolism and cellular processes. More interestingly, nearly 50% of all acetylated proteins identified in this work were localized in chloroplast and the vital role of lysine acetylation in photosynthesis was also revealed. Taken together, this study not only established the most extensive lysine acetylome in plants to date, but also systematically suggests the significant and unique roles of lysine acetylation in plants.