Docosahexaenoic acid (DHA) ameliorates paraquat-induced pulmonary fibrosis in rats possibly through up-regulation of Smad 7 and SnoN
Docosahexaenoic acid (DHA) ameliorates paraquat-induced pulmonary fibrosis in rats possibly through up-regulation of Smad 7 and SnoN
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DOI：
10.1016/j.fct.2013.03.045
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发表时间：
2013-07-01
影响因子：
4.3
通讯作者：
Zhao, Xiulan
中科院分区：
文献类型：
--
作者：
Chen, Jingjing;Zeng, Tao;Zhao, Xiulan
百草枯（Paraquat，PQ）中毒可导致进行性、不可逆的肺纤维化，造成大量人员死亡。二十二碳六烯酸（DHA）是公认的影响健康和疾病的多种生物途径的重要调节剂。一系列研究表明，DHA补充剂与减轻某些组织纤维化有关。在本研究中，通过单次经口给予50 mg/kg bw PQ处理，导致大鼠肺纤维化。在PQ处理前7天提供每日500 mg/kg bw DHA补充剂，并持续连续35天。DHA可改善PQ诱导的肺纤维化改变，其表现为组织学改变、羟脯氨酸含量和转化生长因子-β（1）（TGF-β（1））mRNA水平的显著降低。此外，补充DHA的大鼠中Smad 7和SnoN的蛋白水平与PQ组的大鼠相比显著增加。结果提示，DHA可减轻PQ诱导的肺纤维化，其机制可能与促进Smad 7和SnoN的表达有关。(C)2013爱思唯尔有限公司保留所有权利。
Paraquat (PQ) poisoning has caused a large number of human fatalities due to the progressive and irreversible pulmonary fibrosis. Docosahexaenoic acid (DHA) is well-recognized as important modulators of multiple biological pathways that affect health and disease. A line of studies have shown that DHA supplementation is associated with the alleviation of some tissue fibrosis. In the current study, pulmonary fibrosis of rats was produced by a single oral dose of 50 mg/kg bw PQ treatment. Daily 500 mg/kg bw DHA supplementation was provided 7 days before PQ treatment and lasted for consecutive 35 days. DHA was found to ameliorate the pulmonary fibrotic alterations induced by PQ which was evidenced by significant reduction of histological changes, hydroxyproline content and level of the transforming growth factor-beta(1) (TGF-beta(1)) mRNA. Furthermore, the protein levels of Smad 7 and SnoN in the DHA supplemented rats were significantly increased compared with those in the rats of the PQ group. These results suggested that DHA ameliorated pulmonary fibrosis induced by PQ might be attributed to its enhancement of Smad 7 and SnoN expression. (C) 2013 Elsevier Ltd. All rights reserved.