Osteoblast and osteoclast differentiation in modeled microgravity
Osteoblast and osteoclast differentiation in modeled microgravity
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DOI：
10.1196/annals.1402.033
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发表时间：
2007-01-01
期刊：
影响因子：
--
通讯作者：
Mcdonald, Jay M.
中科院分区：
文献类型：
--
作者：
Saxena, Ritu;Pan, George;Mcdonald, Jay M.
Prolonged microgravity experienced by astronauts is associated with a decrease in bone mineral density. To investigate the effect of microgravity on differentiation of osteoclasts and osteoblasts, we used a NASA-recommended, ground-based, microgravity-simulating system, the Rotary Cell Culture System (RCCS). Using the RCCS, we demonstrated that modeled microgravity (MMG) inhibited osteoblastogenesis and increased adipocyte differentiation in human mesenchymal stem cells incubated under osteogenic,,conditions. This transformation involves reduced RhoA activity and cofilin phosphorylation, disruption of F-actin stress fibers, and decreased integrin signaling through focal adhesion kinase. We have used the system to show that MMG also stimulates osteoclastogenesis. These systems provide the opportunity to develop pharmacological agents that will stimulate osteoblastogenesis and inhibit osteoclastogenesis.
