Differential infection of porcine alveolar macrophage subpopulations by nonopsonized Mycobacterium bovis involves CD14 receptors.
Differential infection of porcine alveolar macrophage subpopulations by nonopsonized Mycobacterium bovis involves CD14 receptors.
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非调理牛分枝杆菌对猪肺泡巨噬细胞亚群的差异感染涉及 CD14 受体。
DOI：
10.1002/jlb.60.2.214
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发表时间：
1996
影响因子：
5.5
通讯作者：
Molitor,TW
中科院分区：
文献类型：
--
作者：
Khanna,KV;Choi,CS;Gekker,G;Peterson,PK;Molitor,TW
世界范围内分枝杆菌感染的死灰复燃引起了人们对分枝杆菌物种发病机制的重新关注。本研究的目的是表征非调理牛分枝杆菌对肺泡巨噬细胞 (AM) 的感染，并阐明 AM 亚群发生差异感染的机制。观察到 AM 亚群对牛分枝杆菌感染的敏感性存在差异，因此最不密集的细胞最不敏感 (21.4 ± 10.7%)，而最密集的细胞最容易感染 (61.8 ± 5.6%)。 AMs 对 CD14 受体染色的百分比显示出类似的差异分布，表达 CD14 的最不致密细胞较少，而表达 CD14 受体表达的最致密细胞的百分比较高。为了研究 CD14 受体在 AM 感染中的作用，将抗 CD14 抗体添加到细胞培养物中。抗 CD14 抗体可阻断牛分枝杆菌 AM 感染高达 60.2%，但同种型对照抗体则不能。这项研究的结果表明，牛分枝杆菌选择性地感染 AM 亚群，特别是 CD14 表达量最大的亚群，CD14 是牛分枝杆菌感染猪 AM 的假定受体机制。白细胞。生物。 60：214-220； 1996年。
The resurgence in mycobacterial infection worldwide has led to renewed attention to the pathogenesis ofMycobacteriumspecies. The purpose of this study was to characterize the infection of alveolar macrophages (AMs) by nonopsonizedMycobacterium bovis, and to elucidate the mechanism by which a differential infection of subpopulations of AM may occur. A difference in susceptiblity toMycobacterium bovisinfection of subpopulations of AMs was observed, such that the least dense cells were the least susceptible (21.4 ± 10.7%) and the most dense cells were the most readily infected (61.8 ± 5.6%). The percentage of AMs staining for CD14 receptors showed a similar differential distribution, with fewer of the least dense cells expressing CD14 and a greater percentage of the most dense cells staining for CD14 receptor expression. To investigate the role of CD14 receptors in the infection of AMs, anti‐CD14 antibody was added to the cell cultures. Infection of AM byMycobacterium boviswas blocked by up to 60.2% by anti‐CD14 antibody but not by isotype control antibody. The results of this study suggest thatMycobacterium bovisselectively infects AM subpopulations, specifically those with the greatest expression of CD14, a putative receptor mechanism forMycobacterium bovisinfection of porcine AM.J. Leukoc. Biol. 60: 214–220; 1996.