Comprehensive monitoring of mitochondrial viscosity variation during different cell death processes by a NIR mitochondria-targeting fluorescence probe.
Comprehensive monitoring of mitochondrial viscosity variation during different cell death processes by a NIR mitochondria-targeting fluorescence probe.
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DOI：
10.1016/j.saa.2023.122602
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发表时间：
2023-03
期刊：
影响因子：
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通讯作者：
jiaojiao Liu;Fancheng Meng;Jia-jia Lv;Min Yang;Yumei Wu;Jie Gao;Junjun Luo;Xinmin Li;Gang Wei;Zeli Yuan;Hongyu Li
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作者：
jiaojiao Liu;Fancheng Meng;Jia-jia Lv;Min Yang;Yumei Wu;Jie Gao;Junjun Luo;Xinmin Li;Gang Wei;Zeli Yuan;Hongyu Li
细胞死亡是多细胞生物的一个基本特征，线粒体在其中起着至关重要的作用。因此，揭示和监测线粒体的微环境对研究细胞死亡过程具有重要意义。在此，线粒体粘度变化的行为，一系列不同的细胞死亡过程中监测与近红外荧光探针FLV。基于可旋转黄花菁荧光团设计了FLV，该荧光团随着环境粘度的增加而呈现选择性和灵敏性的近红外荧光增强响应。用制霉菌素和不同温度孵育的活细胞进行荧光成像实验，结果表明，FLV能够成像线粒体粘度的变化。最后，FLV被应用于监测不同细胞死亡过程中线粒体粘度的变化。结果发现，在凋亡、坏死和自噬过程中，线粒体粘度明显升高;而在铁凋亡诱导剂erastin或RSL 3作用6 h后，未观察到明显的线粒体粘度变化。这些结果表明，FLV是一个可行的工具，用于监测线粒体粘度的变化，并有可能用于诊断线粒体粘度相关的细胞过程和疾病。
Cell death is a fundamental feature of multicellular organisms, in which mitochondria play crucial roles. Therefore, revealing and monitoring the microenvironment of mitochondria are significant to investigate cell death process. Herein, the mitochondrial viscosity variation behaviors of a series of different cell death processes were monitored with a NIR mitochondria-targeting fluorescence probe FLV. FLV was designed based on a rotatable flavylocyanine fluorophore that presented selective and sensitive NIR fluorescence enhancement response with the increase of environmental viscosity. Fluorescence imaging experiments of living cells incubated with nystatin or under different temperature indicated that FLV was capable of imaging the change of mitochondrial viscosity. Finally, FLV was applied for monitoring the mitochondrial viscosity variation during different cell death processes. It was found that there were obvious mitochondrial viscosity increases during apoptosis, necrosis and autophagy; however, no detectable mitochondrial viscosity variation was observed in ferroptosis process incubated with ferroptosis inducer erastin or RSL3 for 6 h. These results demonstrated that FLV is a viable tool for monitoring the mitochondrial viscosity variation and is likely to be used in the diagnosis of the mitochondrial viscosity-associated cell processes and diseases.