VARIETY OF PHENOTYPICAL EXPRESSION OF INTESTINAL MARKER ENZYMES AND MUCIN IN HUMAN STOMACH INTESTINAL METAPLASIA
VARIETY OF PHENOTYPICAL EXPRESSION OF INTESTINAL MARKER ENZYMES AND MUCIN IN HUMAN STOMACH INTESTINAL METAPLASIA
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DOI：
10.1267/ahc.13.499
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发表时间：
1980-01-01
影响因子：
2.4
通讯作者：
KITAOKA, H
中科院分区：
文献类型：
--
作者：
MATSUKURA, N;KAWACHI, T;KITAOKA, H
用TES胶带法将人胃肠化生宏观上分为粘膜表面蔗糖酶和海藻糖酶活性较高的区域(完全型)和粘膜表面蔗糖酶和海藻糖酶活性较低的区域(不完全类型)。用TES胶带对这两种类型的组织进行5次连续冰冻切片。切片进行肠道标志物酶和粘蛋白的组织化学检查，并对化生小管进行组织学检查。在完全型粘膜中，几乎所有化生小管的纹状边缘均呈亮氨酸氨基肽酶(LAP)和碱性磷酸酶(ALP)阳性反应，含有潘氏S细胞。高铁二胺-阿尔辛蓝(HID-AB)染色未见高铁二胺(HID)阳性粘蛋白。在不完全型粘膜中，LAP阳性的化生小管、HID阳性反应和ALP阴性反应是常见的。LAP、ALP呈阴性反应，LAP、ALP呈阳性反应，但HID呈阴性反应。可见杯状细胞，未见潘氏S细胞。在化生小管内可观察到多种肠道标志物酶和粘蛋白的表型表达，尤其是在不完全型肠化生区。
Intestinal metaplasia in human stomach was classified macroscopically into areas with sucrase and trehalase activities on the mucosal surface (complete type) and areas with sucrase but no trehalase activities on the mucosal surface (incomplete type) by the TES-TAPE method. Five serial frozen sections were taken from tissues of these 2 types located with TES-TAPE. The sections were examined histochemically for intestinal marker enzymes and mucin and the metaplastic tubules were also examined histologically. In mucosa from areas of the complete type, the striated border of almost all the metaplastic tubules gave positive reactions for leucine aminopeptidase (LAP) and alkaline phosphatase (ALP) and contained Paneth''s cells. No high-iron diamine (HID) positive mucin was detected by staining with high-iron diamine-Alcian blue (HID-AB). In mucosa from areas of the incomplete type, metaplastic tubules giving a positive reaction for LAP, a HID-positive reaction and a negative reaction for ALP were frequently observed. Other patterns, such as negative reactions for LAP, ALP and positive reactions for LAP and ALP, but a HID-negative reaction was also observed. Goblet cells were present but no Paneth''s cells were found. A variety of phenotypical expressions of intestinal marker enzymes and mucin were observed in metaplastic tubules, especially in areas of the incomplete type of intestinal metaplasia.