Deregulation of Plant Cell Death Through Disruption of Chloroplast Functionality Affects Asexual Sporulation of Zymoseptoria tritici on Wheat
Deregulation of Plant Cell Death Through Disruption of Chloroplast Functionality Affects Asexual Sporulation of Zymoseptoria tritici on Wheat
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DOI：
10.1094/mpmi-10-14-0346-r
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发表时间：
2015-05-01
影响因子：
3.5
通讯作者：
Kanyuka, Kostya
中科院分区：
文献类型：
--
作者：
Lee, Wing-Sham;Devonshire, B. Jean;Kanyuka, Kostya
叶绿体作为水杨酸（SA）、茉莉酸（JA）和一氧化氮（NO）等信号化合物的生物合成场所以及活性氧产生的主要场所，在植物防御中具有关键作用。因此，叶绿体被认为是诱导和调节细胞程序性死亡（PCD）以响应非生物胁迫和病原菌攻击的重要参与者。小麦Zymoseptoria的主要叶面病原体的三孢菌提出利用植物PCD，这是与真菌的感染的necrotrophic阶段的过渡。在这项研究中，病毒诱导的基因沉默被用来沉默类胡萝卜素和叶绿素生物合成的两个关键基因，八氢番茄红素去饱和酶（PDS）和镁螯合酶H亚基（ChlH）。叶绿素缺乏，PDS和ChlH沉默的敏感植物叶片经历了更快的病原体诱导的PCD，但显着不太能够支持随后的无性孢子形成的Z。三个月。相反，主效基因（Stb6）介导的抗白叶枯病抗性。在PDS和ChlH沉默的叶子中，小麦受到部分损害。叶绿素缺乏的小麦穗也表现出增加的Z。伴随着无性孢子形成增加的炭疽病病斑形成。这些数据突出了叶绿体功能的重要性及其与调控植物细胞死亡的相互作用，介导不同基因型和组织特异性之间的相互作用Z。小麦和小麦。
Chloroplasts have a critical role in plant defense as sites for the biosynthesis of the signaling compounds salicylic acid (SA), jasmonic acid (JA), and nitric oxide (NO) and as major sites of reactive oxygen species production. Chloroplasts, therefore, regarded as important players in the induction and regulation of programmed cell death (PCD) in response to abiotic stresses and pathogen attack. The predominantly foliar pathogen of wheat Zymoseptoria tritici is proposed to exploit the plant PCD, which is associated with the transition in the fungus to the necrotrophic phase of infection. In this study virus-induced gene silencing was used to silence two key genes in carotenoid and chlorophyll biosynthesis, phytoene desaturase (PDS) and Mg-chelatase H subunit (ChlH). The chlorophyll-deficient, PDS- and ChlH-silenced leaves of susceptible plants underwent more rapid pathogen-induced PCD but were significantly less able to support the subsequent asexual sporulation of Z. tritici. Conversely, major gene (Stb6)-mediated resistance to Z. tritici was partially compromised in PDS- and ChlH-silenced leaves. Chlorophyll-deficient wheat ears also displayed increased Z. tritici disease lesion formation accompanied by increased asexual sporulation. These data highlight the importance of chloroplast functionality and its interaction with regulated plant cell death in mediating different genotype and tissue-specific interactions between Z. tritici and wheat.