A unique right end-enhancer complex precedes synapsis of Mu ends: the enhancer is sequestered within the transpososome throughout transposition.
A unique right end-enhancer complex precedes synapsis of Mu ends: the enhancer is sequestered within the transpososome throughout transposition.
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独特的右端增强子复合物先于 Mu 末端的突触：在整个转座过程中增强子被隔离在转座体内。
DOI：
10.1093/emboj/cdg354
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
Harshey,RasikaM
中科院分区：
文献类型：
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作者：
Pathania,Shailja;Jayaram,Makkuni;Harshey,RasikaM
Mu转座体的组装依赖于转座酶亚基与Mu的左（L）和右（R）末端以及增强子（E）的相互作用。我们在形成三位点复合物（LER）之前和期间跟踪了这些位点在一系列转座体内缔合的顺序和动力学，转座酶活性位点（0型复合物）接合Mu末端，末端切割（I型复合物）及其转移到靶DNA（II型复合物）。LER之前似乎有一个双位点复合物（ER），其中E和R相互包裹两次，如在成熟的转座体中。在此后的每个阶段，五个DNA超螺旋的整体拓扑结构都被保留下来：两个在E和R之间，一个在E和L之间，两个在L和R之间。然而，LER内的L-R相互作用似乎是灵活的。出乎意料的是，通过Mu末端的切割和链转移到靶DNA，看到增强子在转座体内持续存在。
Assembly of the Mu transpososome is dependent on interactions of transposase subunits with the left (L) and right (R) ends of Mu and an enhancer (E). We have followed the order and dynamics of association of these sites within a series of transpososomes prior to and during formation of a three‐site complex (LER), engagement of Mu ends by the transposase active site (type 0 complex), cleavage of the ends (type I complex) and their transfer to target DNA (type II complex). LER appears to be preceded by a two‐site complex (ER) where E and R are interwrapped twice, as in the mature transpososome. At each stage thereafter, the overall topology of five DNA supercoils is retained: two between E and R, one between E and L and two between L and R. However, L–R interactions within LER appear to be flexible. Unexpectedly, the enhancer was seen to persist within the transpososome through cleavage and strand transfer of Mu ends to target DNA.