DNA abasic site-based aptamer for selective fluorescence light-up detection of fisetin by excited-state intramolecular proton transfer
DNA abasic site-based aptamer for selective fluorescence light-up detection of fisetin by excited-state intramolecular proton transfer
复制标题
基于DNA脱碱基位点的适体，用于通过激发态分子内质子转移选择性荧光照明检测非瑟酮
DOI：
10.1016/j.snb.2012.05.051
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发表时间：
2012-08
期刊：
影响因子：
--
通讯作者：
Wu Fei
中科院分区：
文献类型：
--
作者：
Xu Shujuan;Shao Yong;Ma Kun;Cui Qinghua;Liu Guiying;Wu Fei
由于适体在多种靶点上的广泛应用，人们对适体的开发进行了大量的努力。在这项工作中，我们试图使用一个无碱基位点（AP位点），这是嵌入在一个双链DNA作为非瑟酮的结合口袋。由于AP位点完全相对并侧翼为胸腺嘧啶（称为T-T适体），该适体对非瑟酮表现出高于其他类黄酮如桑色素、芦丁、芹菜素、山奈酚、杨梅素、槲皮素、毛地黄黄酮、黄芩苷、柚皮素、染料木素、白杨素和高良姜素的高结合选择性和敏感性。当与AP位点结合时，非瑟酮通过促进其激发态分子内质子转移（ESIPT）反应的发生而经历荧光发射的显著增强。在信噪比为3时，检测限约为50 nM。因此，实现非瑟酮的选择性分析是有益的，从新的适体设计没有任何荧光基团的修饰。因此，在我们的方法中实现了来自适体的几乎可忽略的荧光背景。
Many efforts have been made for the developments of aptamers due to their wide applications for various targets. In this work, we tried to use an abasic site (AP site) that was embeded in a double-stranded DNA as a binding pocket for fisetin. With the AP site being wholly opposed and flanked by thymines (named T-T aptamer), the aptamer exhibits high binding selectivity and sensitivity for fisetin over the other flavonoids such as morin, rutin, apigenin, kaempferol, myricetin, quercetin, luteolin, baicalin, naringenin, genistein, chrysin, and galangin. Upon binding to the AP site, fisetin experiences a significant enhancement in fluorescence emisson by favoring the occurrence of its excited-state intramolecular proton transfer (ESIPT) reaction. The detection limit is about 50nM at a signal-to-noise ratio of 3. Therefore, the realization for fisetin's selective analysis is beneficial from the novel aptamer design without any fluorophore modification. Thus, the almost negligible fluorescence background from the aptamer is achieved in our method.