Mechanical stimulation of individual stereocilia of living cochlear hair cells by atomic force microscopy
Mechanical stimulation of individual stereocilia of living cochlear hair cells by atomic force microscopy
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DOI：
10.1016/s0304-3991(99)00136-9
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发表时间：
2000-02-01
期刊：
影响因子：
2.2
通讯作者：
Ruppersberg, JP
中科院分区：
文献类型：
--
作者：
Langer, MG;Koitschev, A;Ruppersberg, JP
本文从个体立体纤毛水平对活体内、外毛细胞(IHC)和外毛细胞(OHC)的弹性特性和成像进行了研究。使用了定制的AFM装置，允许在直立的差动干涉对比(DIC)显微镜和浸水物镜的直接控制下扫描内耳的机械敏感结构。AFM研究后获得的相同发束的扫描电子显微镜(SEM)图像表明，高达1.5纳米牛顿(NN)的力不会对立体纤毛的表面形态造成明显破坏。这是第一批由AFM拍摄的Corti器官活体感觉细胞的发束图像，它们显示了个体立体纤毛的尖端和典型的纤毛束的V形。由于线扫描清楚地表明斜率和力的相互作用取决于立体纤毛的弹性性质，因此建议对单个传导通道进行定量的刚度测量和刺激。(C)2000 Elsevier Science B.V.保留所有权利。
This paper describes the investigation of elastical properties and imaging of living cochlear hair bundles of inner (IHC) and outer hair cells (OHC) on the level of individual stereocilia. A custom-made AFM-setup was used, allowing to scan the mechano-sensitive structures of the inner ear under direct control of an upright differential interference contrast (DIC) microscope with a water-immersion objective. Scanning electron microscopy (SEM) images of the identical hair bundles obtained after AFM investigation demonstrated that forces up to 1.5 nanonewton (nN) did not cause obvious damage of the surface morphology of the stereocilia. These are the first images of hair bundles of living sensory cells of the organ of Corti by AFM, They display the tips of individual stereocilia and the typical V-shape of ciliary bundles. Since line scans clearly show that slope and force interaction depend on the elastical properties of stereocilia, quantitative stiffness measurements and stimulation of single transduction channels are suggested. (C) 2000 Elsevier Science B.V. All rights reserved.