Membrane vesicles released by Actinobacillus pleuropneumoniae contain proteases and Apx toxins
Membrane vesicles released by Actinobacillus pleuropneumoniae contain proteases and Apx toxins
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DOI：
10.1111/j.1574-6968.2000.tb09326.x
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发表时间：
2000-10-01
影响因子：
2.1
通讯作者：
de la Garza, M
中科院分区：
文献类型：
--
作者：
Negrete-Abascal, E;García, RM;de la Garza, M
胸膜肺炎放线杆菌血清型1将含有蛋白酶和Apr毒素的囊泡释放到培养基中。囊泡通过超离心浓缩，并通过电子显微镜和电泳进行分析;它们的大小范围从20至200 nm。针对纯化的血清型1高分子量分泌蛋白酶产生的多克隆抗血清通过免疫金电子显微镜识别囊泡表面的这种蛋白酶。从囊泡提取物的较高分子量的多肽被识别的抗血清通过Western免疫印迹，表明蛋白酶可以形成寡聚体。然而，这些低聚物在分泌之前对明胶没有活性。此外，Apx毒素也存在于囊泡中，并通过抗血清型1毒素多克隆血清的Western免疫印迹识别。A.囊泡中的胸膜肺炎抗原被来自感染血清型1或5的动物的恢复期猪血清识别。含有毒力因子的囊泡的释放可能是猪胸膜肺炎的一种组织损伤机制。(C)2000年由Elsevier Science B.V代表欧洲微生物学会联合会出版。
Actinobacillus pleuropneumoniae serotype 1 releases vesicles containing proteases and Apr toxins into the culture medium. Vesicles were concentrated by ultracentrifugation and analyzed by electron microscopy and electrophoresis; their size ranged from 20 to 200 nm. A polyclonal antiserum raised against a purified high molecular mass secreted protease of serotype 1 recognized this protease on the surface of the vesicles by immunogold electron microscopy. Higher molecular mass polypeptides from vesicle extracts were recognized by the antiserum by Western immunoblot, indicating that the protease could form oligomers. However, these oligomers were not active against gelatin until secreted. Additionally, Apx toxins were also present in vesicles, and were recognized by Western immunoblot by an anti-serotype 1 toxins polyclonal serum. A. pleuropneumoniae antigens in vesicles were recognized by convalescent-phase pig sera from animals infected with serotype 1 or 5. The release of vesicles containing virulence factors could be a tissue damage mechanism in swine pleuropneumonia. (C) 2000 Published by Elsevier Science B.V, on behalf of the Federation of European Microbiological Societies.