A Strategy to Identify Genes Associated with Circulating Solid Tumor Cell Survival in Peripheral Blood
A Strategy to Identify Genes Associated with Circulating Solid Tumor Cell Survival in Peripheral Blood
复制标题
鉴定与外周血中循环实体瘤细胞存活相关的基因的策略
DOI：
10.1007/bf03402067
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发表时间：
1999
影响因子：
5.7
通讯作者：
A. Pardee
中科院分区：
文献类型：
--
作者：
M. Fournier;Maria Gloria Costa Carvalho;A. Pardee
转移研究的努力集中在原发部位和转移部位肿瘤之间的表型和遗传差异上。然而，可用作循环肿瘤细胞中转移的分子标志物的基因仍然未被鉴定。调节血液中实体瘤细胞的传播和存活以及它们对新环境的适应的基因可能是独特的转移性肿瘤标志物的候选者。进行差异显示（DD）以比较无肿瘤个体的血液与肺癌、乳腺癌和结肠癌患者的血液。在肿瘤患者血液样品中鉴定出21个上调表达的基因，但在无肿瘤供体血液样品中没有。这些样品中的9个被分离、扩增并直接测序。AB-1基因同源的Bcl-2家族成员，这可能是作为一个凋亡抑制剂，被确定。转移性肿瘤患者血液中凋亡抑制因子的过度表达可能与实体瘤细胞在外周血中存活的能力相关。这是通过DD比较对照和患者血液样品以发现识别血液中转移的新的潜在遗传标记的有用性的首次证明。
Efforts in metastasis research have centered on the phenotypic and genetic differences between primary site and metastatic site tumors. However, genes that may be used as molecular markers of metastasis in circulating tumor cells remain unidentified. Genes regulating the dissemination and survival of solid tumor cells in the blood, as well as their adaptation to new environments, could be candidates for unique metastatic tumor markers. Differential display (DD) was conducted to compare the blood of tumor-free individuals with the blood of patients with lung, breast, and colon cancers. Twenty-one up-expressed genes in the tumor patient blood samples but none in the tumor-free donor blood samples were identified. Nine of these samples were isolated, amplified, and directly sequenced. A gene AB-1 homologous to a Bcl-2 family member, which might function as an apoptosis inhibitor, was identified. The overexpression of an apoptosis inhibitor in blood from patients with metastatic tumors might be correlated with the capability of solid tumor cells to survive in peripheral blood. This is the first demonstration of the usefulness of comparing control and patient blood samples by DD to find novel potential genetic markers identifying metastasis in the blood.
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新的引物策略提高了差异显示的精度。
DOI：
--
发表时间：
1995
期刊：
BioTechniques.
影响因子：
--
作者：
Zhao,S;Ooi,SL;Pardee,AB
通讯作者：
Pardee,AB
影响因子：
11.2
作者：
Zhongyun Dong;A. Staroselsky;Xiaoxia Qi;Keping Xie;I. J. Fidler
通讯作者：
Zhongyun Dong;A. Staroselsky;Xiaoxia Qi;Keping Xie;I. J. Fidler
影响因子：
14.9
作者：
LIANG, P;AVERBOUKH, L;PARDEE, AB
通讯作者：
PARDEE, AB
人 Bcl-2 同源物的克隆：炎性细胞因子在培养的内皮细胞中诱导人 A1。
影响因子：
20.3
作者：
Karsan,A;Yee,E;Kaushansky,K;Harlan,JM
通讯作者：
Harlan,JM