Maternal protein restriction regulates IGF2 system in placental labyrinth.
Maternal protein restriction regulates IGF2 system in placental labyrinth.
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DOI：
10.2741/472
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发表时间：
2012
期刊：
影响因子：
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通讯作者：
Haijun Gao;K. Sathishkumar;U. Yallampalli;Meena P Balakrishnan;Xilong Li;Guoyao Wu;C. Yallampalli
中科院分区：
文献类型：
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作者：
Haijun Gao;K. Sathishkumar;U. Yallampalli;Meena P Balakrishnan;Xilong Li;Guoyao Wu;C. Yallampalli
本研究旨在验证胎盘迷宫区（LZ） IGF2系统的改变对胎儿胎盘生长的影响是对母体蛋白限制的反应。大鼠在妊娠第1 ~ 14、18、21天分别饲喂20%蛋白质饲料和6%等热量蛋白质饲料（LP）。分析日粮、胎盘和胎儿性别、妊娠天数对胎盘重、胎儿重、胎盘LZ中IGF2轴表达及母体血浆氨基酸的影响。LP对女性和男性胎儿均产生生长限制，与LZ生长和效率受损相一致。胎盘LZ中Igf2、Igf2P0、Igf1r、Igf2r、Insr、Igfbp1和Igfbp2的表达受饮食、性别和/或妊娠天数的影响。饲喂lp的大鼠母体血浆中总必需氨基酸和总非必需氨基酸浓度分别降低和升高。这些结果表明，IGF2系统在大鼠LZ中的适应以性别和时间依赖的方式发生，以响应母体蛋白限制；然而，这些适应并不能防止男性和女性胎儿在妊娠后期的生长限制。
This study was to test the hypothesis that altered IGF2 system in the placental labyrinth zone (LZ) impairs feto-placental growth in response to maternal protein restriction. Rats were fed a 20% protein diet and an isocaloric 6 % protein diet (LP) from day 1 to days 14, 18, or 21 of pregnancy. The effects of diet, gender of placenta and fetus, and day of pregnancy on placental weight, fetal weight, and expression of the IGF2 axis in the placental LZ and amino acids in maternal plasma were analyzed. Growth restriction occurred in both female and male fetuses by LP, coincident with impaired LZ growth and efficiency. The expression of Igf2, Igf2P0, Igf1r, Igf2r, Insr, Igfbp1, and Igfbp2 in placental LZ were affected by diet, gender and/or day of pregnancy. Concentrations of total essential amino acids and total nonessential amino acids were reduced and increased, respectively, in maternal plasma of LP-fed rats. These results indicate that adaptation of the IGF2 system in rat LZ occurs in a sex- and time-dependent manner in response to maternal protein restriction; however, these adaptations cannot prevent the growth restriction of both male and female fetuses during late pregnancy.