Stress-induced increase of extracellular levels of cyclic AMP in rat cortex.
Stress-induced increase of extracellular levels of cyclic AMP in rat cortex.
复制标题
应激诱导大鼠皮层细胞外环磷酸腺苷水平增加。
DOI：
10.1016/0006-8993(92)91516-h
复制
发表时间：
1992
期刊：
影响因子：
2.9
通讯作者：
John,SM
中科院分区：
文献类型：
--
作者：
Stone,EA;John,SM
以前的研究表明，压力可能会增加大脑中的环磷酸腺苷（cAMP）水平，但这些发现一直存在争议，因为使用压力程序来抑制大脑酶。因此，本实验采用非应激技术，微透析，测定细胞外cAMP水平在大鼠皮质应激后。在额叶皮层中植入探针后2天进行实验。显着增加后，发现约束或腹膜内注射生理盐水的轻度应激，这表明增加组织水平的cAMP已经发生。这些反应被增强的磷酸二酯酶（PDE）抑制剂，咯利普兰的局部输液。它的结论是，一个或多个腺苷酸环化酶偶联受体在皮层被激活的轻度应力，这种激活可以在体内检测微透析。
Previous studies have suggested that stress may increase levels of cyclic AMP (cAMP) in the brain but these findings have been controversial due to the use of stressful procedures to inactivate brain enzymes. The present experiment therefore used a non-stressful technique, microdialysis, to assay extracellular levels of cAMP in the rat cortex after stress. Experiments were conducted 2 days after implantation of probes in the frontal cortex. Significant increases were found after the mild stressors of restraint or intraperitoneal injection of saline suggesting that increased tissue levels of cAMP had occurred. These responses were potentiated by local infusion of the phosphodiesterase (PDE) inhibitor, rolipram. It is concluded that one or more adenylate cyclase-coupled receptors in the cortex is activated by mild stress and that this activation can be detected in vivo by microdialysis.