Isolation of (a subunit of) the Na+/D‐glucose contransporter(s) of rabbit intestinal brush border membranes using monoclonal antibodies
Isolation of (a subunit of) the Na+/D‐glucose contransporter(s) of rabbit intestinal brush border membranes using monoclonal antibodies
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使用单克隆抗体分离兔肠刷状缘膜的 Na+/D-葡萄糖转运蛋白（的亚基）
DOI：
10.1016/0014-5793(83)81025-4
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发表时间：
1983
期刊：
影响因子：
3.5
通讯作者：
G. Semenza
中科院分区：
文献类型：
--
作者：
U. Schmidt;B. Eddy;C. Fraser;J. Craig Venter;G. Semenza
从小肠或肾刷状缘膜中鉴定和分离Na+/D-葡萄糖协同转运蛋白已被证明是一项艰巨的任务。过去已经使用了许多方法，包括溶解-重构光亲和性和差异标记，但这些技术的成功有限。我们在这里报告的单克隆抗体的纯化Na+/D-葡萄糖协同转运体从兔小肠刷状缘膜的成功使用。
The identification and isolation of the Na+/D-glucose cotransporter (s) from either smallintestinal or renal brush border membranes has proven to be a formidable task. A number of approaches have been used in the past, including solubilization-reconstitution photoaffinity and differential labelling but these techniques have been of limited success. We here report the successful use of a monoclonal antibody for the purification of the Na+/D-glucose cotransporter from rabbit small-intestinal brush border membranes.