Phylogeny and PCR-based classification of Wolbachia strains using wsp gene sequences
Phylogeny and PCR-based classification of Wolbachia strains using wsp gene sequences
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DOI：
10.1098/rspb.1998.0324
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发表时间：
1998-03-22
影响因子：
4.7
通讯作者：
O'Neill, S
中科院分区：
文献类型：
--
作者：
Zhou, WG;Rousset, F;O'Neill, S
沃尔巴克氏菌是一组细胞内遗传细菌，广泛侵染节肢动物。它们与寄主中许多不同的生殖表型有关，如细胞质不亲和、孤雌生殖和雌性化。虽然已知负责这些不同寄主表型的细菌菌株在CL-变形杆菌中形成一个单一分支，但到目前为止还不可能解决不同沃尔巴克氏菌菌株之间的进化关系。为了解决这个问题，我们从28株沃尔巴克氏菌的代表性样本中克隆并测序了一个编码沃尔巴克氏菌表面蛋白(WSP)的基因。该基因序列变异较大，可用于沃尔巴克氏菌不同菌株的系统发育关系研究。根据沃尔巴克氏菌不同分离株的wsp基因序列，我们初步将其划分为12个类群。随着更多的序列信息可用，我们预计这类群的数量将会增加。此外，我们提出了一种基于群特异性WSP聚合酶链式反应(PCR)引物的沃尔巴克氏菌分类方法，该方法将允许沃尔巴克氏菌分离株进行分型，而不需要克隆和测序单个沃尔巴克氏菌基因。该系统应有助于今后从不同寄主物种的大样本中调查沃尔巴克氏菌菌株的分布和生物学。
Wolbachia are a group of intracellular inherited bacteria chat infect a wide range of arthropods. They are associated with a number of different reproductive phenotypes in their hosts, such as cytoplasmic incompatibility, parthenogenesis and feminization. While it is known that the bacterial strains responsible for these different host phenotypes form a single clade within the cl-Proteobacteria, until now it has not been possible to resolve the evolutionary relationships between different Wolbachia strains. To address this issue we have cloned and sequenced a gene encoding a surface protein of Wolbachia (wsp) from a representative sample of 28 Wolbachia strains. The sequences from this gene were highly variable and could be used to resolve the phylogenetic relationships of different Wolbachia strains. Based on the sequence of the wsp gene from different Wolbachia isolates we propose that the Wolbachia pipientis clade be initially divided into 12 groups. As more sequence information becomes available we expect the number of such groups to increase. In addition, we present a method of Wolbachia classification based on the use of group-specific wsp polymerase chain reaction (PCR) primers which will allow Wolbachia isolates to be typed without the need to clone and sequence individual Wolbachia genes. This system should facilitate future studies investigating the distribution and biology of Wolbachia strains from large samples of different host species.