In Vivo Time-Lapse Imaging and Serial Section Electron Microscopy Reveal Developmental Synaptic Rearrangements
In Vivo Time-Lapse Imaging and Serial Section Electron Microscopy Reveal Developmental Synaptic Rearrangements
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DOI：
10.1016/j.neuron.2010.12.022
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发表时间：
2011-01-27
期刊：
影响因子：
16.2
通讯作者：
Cline, Hollis
中科院分区：
文献类型：
--
作者：
Li, Jianli;Erisir, Alev;Cline, Hollis
树突、轴突和突触在电路发育过程中是动态的；然而，当分支稳定时，微电路连接的变化尚未被直接证明。通过结合爪蟾顶盖神经元的体内延时成像和成像神经元的电镜重建，我们报告了单个脊椎动物神经元上突触的分布和超微结构，并将这些突触特性与数小时或数天成像后树突和轴突乔木结构的动力学联系起来。动态树突具有高密度的未成熟突触，而稳定树突具有较少的成熟突触。轴突通过稳定分支上的多突触钮发起接触。通过减少从多个输入到突触后树突的收敛性和减少从多突触钮扣到突触后位点的发散性，连接得以完善。视觉剥夺或NMDAR拮抗剂减少了突触的成熟和消除，这表明协同输入活动通过同时调节剩余接触突触的消除和成熟来促进微电路的发育。
Dendrites, axons, and synapses are dynamic during circuit development; however, changes in microcircuit connections as branches stabilize have not been directly demonstrated. By combining in vivo time-lapse imaging of Xenopus tectal neurons with electron microscope reconstructions of imaged neurons, we report the distribution and ultrastructure of synapses on individual vertebrate neurons and relate these synaptic properties to dynamics in dendritic and axonal arbor structure over hours or days of imaging. Dynamic dendrites have a high density of immature synapses, whereas stable dendrites, have sparser, mature synapses. Axons initiate contacts from multisynapse boutons on stable branches. Connections are refined by decreasing convergence from multiple inputs to postsynaptic dendrites and by decreasing divergence from multisynapse boutons to postsynaptic sites. Visual deprivation or NMDAR antagonists decreased synapse maturation and elimination, suggesting that coactive input activity promotes microcircuit development by concurrently regulating synapse elimination and maturation of remaining contacts.