TRIMETHYLPSORALEN INDUCES SMALL DELETION MUTATIONS IN CAENORHABDITIS-ELEGANS
TRIMETHYLPSORALEN INDUCES SMALL DELETION MUTATIONS IN CAENORHABDITIS-ELEGANS
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DOI：
10.1073/pnas.91.4.1381
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发表时间：
1994-02-15
影响因子：
11.1
通讯作者：
WOOD, WB
中科院分区：
文献类型：
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作者：
YANDELL, MD;EDGAR, LG;WOOD, WB
为了研究4，5 '，8-三甲基补骨脂素（TMP）对秀丽隐杆线虫的致突变谱，我们分离了TMP致突变后unc-22和pal-1基因的突变，并通过Southern印迹分析了它们的限制性片段长度多态性。21个unc-22突变中有11个表现出限制性片段长度多态性，其中8个为0.10 ~ 15 kb长度的缺失。两个pal-1突变也都是在这个大小范围内的小缺失。我们的结果与之前关于γ射线和X射线致突变的研究的比较表明，TMP的致突变谱可能相似。TMP应可用于产生导致单个基因功能完全丧失并可能导致等位基因特异性DNA多态性的突变。
To examine the mutagenic spectrum of 4,5',8-trimethylpsoralen (TMP) in Caenorhabditis elegans, we isolated mutations in the unc-22 and pal-1 genes following TMP mutagenesis and analyzed them for restriction fragment length polymorphisms by Southern blot. Eleven of 21 unc-22 mutations exhibited restriction fragment length polymorphisms, 8 of which were deletions of between 0.10 and 15 kb in length. Both of two pal-1 mutations were also small deletions within this size range. Comparison of our results with previous studies on mutagenesis by gamma-rays and x-rays suggests that the mutagenic spectrum of TMP may be similar. TMP should be useful in generating mutations that cause complete loss of function of single genes and that are likely to result in allele-specific DNA polymorphisms.