Dynamic coupling between actin network flow and turnover revealed by flow mapping in the lamella of crawling fragments.
Dynamic coupling between actin network flow and turnover revealed by flow mapping in the lamella of crawling fragments.
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DOI：
10.1016/j.bbrc.2009.10.052
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发表时间：
2009-12
影响因子：
3.1
通讯作者：
K. Okeyo;T. Adachi;M. Hojo
中科院分区：
文献类型：
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作者：
K. Okeyo;T. Adachi;M. Hojo
肌动蛋白丝网络的动态翻转和运输是细胞迁移过程中产生张力和牵引力的必要条件。为了阐明肌动蛋白网络流动和营业额之间的动态耦合，我们专注于最简单但优雅的运动系统之一的板层中的流动动力学;来自鱼类角膜细胞的爬行片段。有趣的是，我们发现，在片层的片段肌动蛋白网络是不固定的，如前所述，但表现出的流动动力学，是惊人的相似，报告的高阶细胞，这表明网络流是一个内在的特性的肌动蛋白细胞骨架，是细胞迁移的基础。我们还表明，而聚合介导的网络组装在前面，令人惊讶的是，网络流收敛调制网络拆卸向后方的薄层，这表明流动和营业额耦合在迁移过程中。使用简单的运动系统获得的这些结果是重要的迁移细胞中的肌动蛋白网络动力学的理解，他们将被发现有用的生物物理模型，阐明细胞迁移的基本机制。
Dynamic turnover and transport of actin filament network is essential for protrusive force generation and traction force development during cell migration. To elucidate the dynamic coupling between actin network flow and turnover, we focused on flow dynamics in the lamella of one of the simplest but elegant motility systems; crawling fragments derived from fish keratocytes. Interestingly, we show that actin network in the lamella of fragments is not stationary as earlier reported, but exhibits a flow dynamics that is strikingly similar to that reported for higher order cells, suggesting that network flow is an intrinsic property of the actin cytoskeleton that is fundamental to cell migration. We also demonstrate that whereas polymerization mediates network assembly at the front, surprisingly, network flow convergence modulates network disassembly toward the rear of the lamella, suggesting that flow and turnover are coupled during migration. These results obtained using simple motility systems are significant to the understanding of actin network dynamics in migrating cells, and they will be found useful for developing biophysical models for elucidating the fundamental mechanisms of cell migration.