Impact of antibiotics on expression of virulence-associated exotoxin genes in methicillin-sensitive and methicillin-resistant Staphylococcus aureus
Impact of antibiotics on expression of virulence-associated exotoxin genes in methicillin-sensitive and methicillin-resistant Staphylococcus aureus
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DOI：
10.1086/510396
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发表时间：
2007-01-15
影响因子：
6.4
通讯作者：
Bryant, Amy E.
中科院分区：
文献类型：
--
作者：
Stevens, Dennis L.;Ma, Yongsheng;Bryant, Amy E.
细胞外蛋白质毒素有助于多种金黄色葡萄球菌感染的发病机制。本研究调查了细胞壁活性抗生素和蛋白质合成抑制剂对甲氧西林敏感和耐甲氧西林的S.金黄色。亚抑制浓度的萘夫西林诱导并延长了Panton-Valentine杀白细胞素、α毒素和中毒性休克综合征毒素1的mRNA，并增加了毒素的产生。相比之下，克林霉素和利奈唑胺显着抑制翻译，但不转录，毒素基因。这些结果表明：（1）蛋白质合成抑制是选择抗菌药物治疗产毒素革兰氏阳性病原体引起的严重感染的重要考虑因素;（2）通过诱导和增强毒素产生，无意中使用β-内酰胺类抗生素治疗耐甲氧西林链球菌。金黄色葡萄球菌感染可能导致更差的结果。
Extracellular protein toxins contribute to the pathogenesis of a wide variety of Staphylococcus aureus infections. The present study investigated the effects that cell-wall active antibiotics and protein-synthesis inhibitors have on transcription and translation of genes for Panton-Valentine leukocidin, alpha-hemolysin, and toxic-shock syndrome toxin 1, in both methicillin-sensitive and methicillin-resistant S. aureus. Subinhibitory concentrations of nafcillin induced and prolonged mRNA for Panton-Valentine leukocidin, alpha-toxin, and toxic-shock syndrome toxin 1 and increased toxin production. In contrast, clindamycin and linezolid markedly suppressed translation, but not transcription, of toxin genes. These results suggest ( 1) that protein-synthesis inhibition is an important consideration in the selection of antimicrobial agents to treat serious infections caused by toxin-producing gram-positive pathogens and ( 2) that, by inducing and enhancing toxin production, inadvertent use of beta-lactam antibiotics to treat methicillin-resistant S. aureus infections may contribute to worse outcomes.