Downregulation of microRNA 99a in oral squamous cell carcinomas contributes to the growth and survival of oral cancer cells
Downregulation of microRNA 99a in oral squamous cell carcinomas contributes to the growth and survival of oral cancer cells
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口腔鳞状细胞癌中 microRNA 99a 的下调有助于口腔癌细胞的生长和存活
DOI：
10.3892/mmr.2012.971
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发表时间：
2012-09-01
影响因子：
3.4
通讯作者：
Wang, Qingqing
中科院分区：
文献类型：
--
作者：
Yan, Bin;Fu, Qihong;Wang, Qingqing
microRNAs (miRNAs) are small non-coding RNA molecules that regulate gene expression by either translational inhibition or mRNA degradation. miRNAs play pivotal roles in physiological functioning and pathological progression. The function of microRNA-99a (miR-99a) in oral squamous cell carcinoma (OSCC) remains unclear. In the present study, we investigated the roles of miR-99a in OSCC development and the underlying mechanisms in 25 cases of primary OSCC tissues and Tca-8113 cells. The cells were analyzed using FACS analysis and western blotting. Results showed that the expression levels of miR-99a were markedly decreased in OSCC tissues compared with the adjacent non-tumor tissues (n=25). The results of in vitro experiments showed that miR-99a mimics significantly inhibited the proliferation of Tca-8113 cells, a tongue squamous carcinoma cell I me, and that miR-99a mimics markedly induced the apoptosis of Tca-8113 cells. Furthermore, we demonstrated that mammalian target of rapamycin (mTOR) was directly targeted by miR-99a, as the overexpression of miR-99a in Tca-8113 cells downregulated the protein expression level of mTOR. Thus, our findings suggest that the downregulation of miR-99a in OSCC tissues is associated with tumor development. miR-99a regulates the growth and survival of OSCC cells and may be exploited as a biomarker and therapeutic target for patients with OSCC.
