Generation and Experimental Outcomes of Closed Femoral Fracture in Mice.
Generation and Experimental Outcomes of Closed Femoral Fracture in Mice.
复制标题
小鼠闭合性股骨骨折的产生和实验结果。
DOI：
10.1007/978-1-0716-0989-7_13
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Drissi,Hicham
中科院分区：
文献类型：
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作者：
Roberts,JosephL;Kinter,ChristopherW;Drissi,Hicham
骨折愈合需要许多细胞类型、生长因子和细胞因子的整合，这些细胞类型、生长因子和细胞因子不能使用体外和计算机模型进行充分研究。这促使了高度信息化的体内动物模型的发展，以了解骨折修复的复杂性。在这里，我们描述了一个修改后的程序，小鼠，首先开发的大鼠Bonnarens和艾因霍恩，不需要皮肤切口或剥离。该手术包括用25号针在股骨髁的皮肤和关节面上钻孔，用克氏针固定，并使用三点弯曲骨折器械创建横向中段骨干骨折。骨折愈合可以使用多种技术进行评估，包括显微计算机断层扫描、扭转试验、组织学和组织形态学分析以及基因表达评估。这种小鼠股骨骨折模型有许多骨科创伤应用，从评估新疗法的安全性和有效性到特定基因对骨修复的影响。
Fracture healing requires the integration of many cell types, growth factors, and cytokines that cannot be adequately studied using in vitro and in silico models. This has prompted the development of highly informative in vivo animal models to understand the complexities of fracture repair. Here, we describe a modified procedure for mice, first developed for rats by Bonnarens and Einhorn, that does not require a skin incision or suturing. This procedure involves boring a hole through the skin and articular surface of the femoral condyle with a 25-gauge needle, fixation with a K-wire, and creation of a transverse mid-diaphyseal fracture using a three-point bending fracture device. Fracture healing can be assessed using a variety of techniques, including microcomputed tomography, torsion testing, histological and histomorphometric analyses, and assessment of gene expression. There are many orthopedic trauma applications of this murine femoral fracture model ranging from assessment of safety and efficacy of novel therapeutics to the influence of specific genes on bone repair.