Characterization of the soybean GmALMT family genes and the function of GmALMT5 in response to phosphate starvation
Characterization of the soybean GmALMT family genes and the function of GmALMT5 in response to phosphate starvation
复制标题
大豆 GmALMT 家族基因的特征以及 GmALMT5 响应磷酸盐饥饿的功能
DOI：
10.1111/jipb.12604
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发表时间：
2018-03-01
影响因子：
11.4
通讯作者：
Liao, Hong
中科院分区：
文献类型：
--
作者：
Peng, Wenting;Wu, Weiwei;Liao, Hong
植物根系分泌苹果酸是提高难溶态磷利用率的一个潜在机制，这主要是由植物ALMT基因家族介导的。在本研究中，在大豆基因组中总共鉴定了34个GmALMT基因。GmALMT的表达模式分歧相当大，在响应磷酸盐（Pi）饥饿在叶，根和花，与表达改变P的可用性在26的34 GmALMT。一个根特异性的GmALMT，其表达显着增强Pi饥饿，GmALMT 5，进行了更详细的研究，以确定其可能的作用，在大豆磷营养。GmALMT 5的组织表达模式，亚细胞定位，和苹果酸分泌从过表达GmALMT 5的转基因大豆毛状根的分析表明，GmALMT 5是一种质膜蛋白，介导的苹果酸流出根。此外，当以微溶性Ca-P作为外源P源时，过表达GmALMT 5的转基因拟南芥的生长和P含量均显著增加。总之，这些结果表明，大豆GmALMT基因家族的成员表现出不同的反应，磷饥饿。该家族的一个成员，GmALMT 5，可能有助于大豆磷效率，提高利用难溶性磷源在磷限制条件下。
A potential mechanism to enhance utilization of sparingly soluble forms of phosphorus (P) is the root secretion of malate, which is mainly mediated by the ALMT gene family in plants. In this study, a total of 34 GmALMT genes were identified in the soybean genome. Expression patterns diverged considerably among GmALMTs in response to phosphate (Pi) starvation in leaves, roots and flowers, with expression altered by P availability in 26 of the 34 GmALMTs. One root-specific GmALMT whose expression was significantly enhanced by Pi-starvation, GmALMT5, was studied in more detail to determine its possible role in soybean P nutrition. Analysis of GmALMT5 tissue expression patterns, subcellular localization, and malate exudation from transgenic soybean hairy roots overexpressing GmALMT5, demonstrated that GmALMT5 is a plasma membrane protein that mediates malate efflux from roots. Furthermore, both growth and P content of transgenic Arabidopsis overexpressing GmALMT5 were significantly increased when sparingly soluble Ca-P was used as the external P source. Taken together, these results indicate that members of the soybean GmALMT gene family exhibit diverse responses to Pi starvation. One member of this family, GmALMT5, might contribute to soybean P efficiency by enhancing utilization of sparingly soluble P sources under P limited conditions.