Neuronal receptors display cytoskeleton-independent directed motion on the plasma membrane
Neuronal receptors display cytoskeleton-independent directed motion on the plasma membrane
复制标题
神经元受体在质膜上表现出不依赖于细胞骨架的定向运动
DOI：
10.1101/269381
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Taylor R
中科院分区：
文献类型：
--
作者：
Taylor R
通常认为跨膜蛋白的定向转运通过细胞内转运囊泡发生。然而，使用单粒子跟踪在大鼠海马神经元与pH敏感的量子点探针，专门报告受体的表面运动，我们已经确定了一个亚群的神经元EphB2受体表现出直接运动之间的突触质膜本身。这种受体运动独立于细胞骨架发生，但依赖于胆固醇，并受神经元活动的调节。
Directed transport of transmembrane proteins is generally believed to occur via intracellular transport vesicles. However, using single-particle tracking in rat hippocampal neurons with a pH-sensitive quantum dot probe that specifically reports surface movement of receptors, we have identified a subpopulation of neuronal EphB2 receptors that exhibit directed motion between synapses within the plasma membrane itself. This receptor movement occurs independently of the cytoskeleton but is dependent on cholesterol and is regulated by neuronal activity.