EVIDENCE FOR AN OLFACTORY RECEPTOR WHICH RESPONDS TO NICOTINE - NICOTINE AS AN ODORANT
EVIDENCE FOR AN OLFACTORY RECEPTOR WHICH RESPONDS TO NICOTINE - NICOTINE AS AN ODORANT
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DOI：
10.1007/bf01951644
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发表时间：
1987-08-15
期刊：
影响因子：
--
通讯作者：
DODD, GH
中科院分区：
文献类型：
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作者：
EDWARDS, DA;MATHER, RA;DODD, GH
烟草生物碱(S)(-)-尼古丁，当作为蒸汽应用于体外头部制剂时，刺激三种大鼠的嗅上皮，在两种小鼠中刺激程度较小。尼古丁产生的电嗅觉图（EOG）与已知气味剂产生的电嗅觉图具有相似的特征。烟碱EOG随烟碱蒸气浓度（1 ~ 100 nM）的增加而增加。在Wistar大鼠和Lister hood大鼠中观察到凝集素刀豆蛋白A对尼古丁EOG的不同降低。通过在凝集素培养基中加入-甲基- d -甘露糖甙，可以防止刀豆蛋白A对尼古丁EOG的还原。这表明至少有一种对尼古丁有反应的嗅觉受体与糖基团有关。我们的研究结果表明，大鼠嗅上皮具有尼古丁受体位点。尼古丁是一种不寻常的化合物，因为它既有气味又有药理特性。
The tobacco alkaloid (S)(-)-nicotine, when applied as a vapour to an in vitro head preparation, stimulates the olfactory epithelium in three strains of rats and to a lesser extent in two strains of mice. The electro-olfactogram (EOG) generated by nicotine has similar characteristics to the EOGs produced by known odorants. The nicotine EOG increases with increasing concentration of nicotine vapour (1-100 nM) applied to the olfactory epithelium. Differential reduction of the nicotine EOG by the lectin concanavalin A is seen in Wistar and Lister Hooded rats. The reduction of the nicotine EOG by concanavalin A is prevented by adding alpha-methyl-D-mannoside to the lectin superfusion medium. This suggests that there is a glyco-moiety associated with at least one olfactory receptor responding to nicotine.Our results suggest that rat olfactory epithelium has receptor sites for nicotine. Nicotine is an unusual compound because it shows both odorant and pharmacological properties.