RNA editing enzyme APOBEC1 and some of its homologs can act as DNA mutators
RNA editing enzyme APOBEC1 and some of its homologs can act as DNA mutators
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DOI：
10.1016/s1097-2765(02)00742-6
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发表时间：
2002-11-01
期刊：
影响因子：
16
通讯作者：
Neuberger, MS
中科院分区：
文献类型：
--
作者：
Harris, RS;Petersen-Mahrt, SK;Neuberger, MS
APOBEC1是RNA编辑复合物的催化组分，但与活化诱导的胞苷脱氨酶（AID）具有同源性，AID是一种功能是增强免疫球蛋白基因DNA多样化的蛋白质。在此，我们发现APOBEC1及其同源物APOBEC3C和APOBEC3G在大肠杆菌中表现出强的DNA突变活性。coli检测。事实上，像AID一样，这些蛋白质似乎通过dC脱氨基作用触发DNA突变。然而，每种蛋白质表现出不同的局部靶序列特异性。结果揭示了一个潜在的活性dC/dG突变体家族的存在，可能与癌症有关。
APOBEC1 is the catalytic component of an RNA editing complex but shows homology to activation-induced cytidine deaminase (AID), a protein whose function is to potentiate diversification of immunoglobulin gene DNA. Here, we show that APOBEC1 and its homologs APOBEC3C and APOBEC3G exhibit potent DNA mutator activity in an E. coli assay. Indeed, like AID, these proteins appear to trigger DNA mutation through dC deamination. However, each protein exhibits a distinct local target sequence specificity. The results reveal the existence of a family of potential active dC/dG mutators, with possible implications for cancer.