Tensile mechanical properties of bovine articular cartilage: variations with growth and relationships to collagen network components
Tensile mechanical properties of bovine articular cartilage: variations with growth and relationships to collagen network components
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DOI：
10.1016/s0736-0266(03)00030-5
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发表时间：
2003-09-01
影响因子：
2.8
通讯作者：
Sah, RL
中科院分区：
文献类型：
--
作者：
Williamson, AK;Chen, AC;Sah, RL
修复关节缺损的一种方法是通过再现胎儿和出生后生长到成年期间发生的变化来再生软骨，从而恢复功能性生物力学特性，特别是那些正常强壮的表面区域。本研究的目的是：(1)表征和比较不同生长阶段（孕晚期胎儿、1-3周龄犊牛和成年牛）的髌股沟（PFG）和股骨髁（FC）关节软骨表面区域的拉伸生物力学特性，以及(2)确定这些特性是否与胶原网络成分相关。从胎儿发育到成人，软骨试样的平衡、动态拉伸模量和强度平均增加391 ~ 1060%，破坏时的应变平均下降43%。胶原浓度（每湿重）提高了98%，吡啶啉交联浓度提高了730%，而糖胺聚糖浓度保持不变或略有下降。一些生长相关的变化是特定于位置的，PFG的拉伸模量和强度高于FC。与生长相关的拉伸模量和强度变化与胶原蛋白和吡啶交联的含量变化密切相关，而与硫代糖胺聚糖含量变化无关。关节软骨的拉伸性能和胶原网络成分随着生长的显著变化表明，这些参数可以用来评估再生软骨再现正常发育和生长的程度。(C) 2003骨科研究学会。Elsevier Ltd.出版。版权所有。
One approach to repairing articular defects is to regenerate cartilage by recapitulating the changes that occur during fetal and postnatal growth into adulthood, and to thereby restore functional biomechanical properties, especially those of the normally strong superficial region. The objectives of this study were (1) to characterize and compare tensile biomechanical properties of the superficial region of articular cartilage of the patellofemoral groove (PFG) and femoral condyle (FC) from bovine animals over a range of growth stages (third-trimester fetal, 1-3 week-old calf, and adult), and (2) to determine if these properties were correlated with collagen network components. With growth from the fetus to the adult, the equilibrium and dynamic tensile moduli and strength of cartilage sample's increased by an average of 391-1060%, while the strain at the failure decreased by 43%. The collagen concentration (per wet weight) increased by 98%, and the pyridinoline cross-link concentration increased by 730%, while the glycosaminoglycan concentration remained unchanged or decreased slightly. Some growth-associated changes were location-specific, with tensile moduli and strength attaining higher values in the PFG than the FC. The growth-associated variation in tensile moduli and strength were associated strongly with variation in the contents of collagen and pyridinoline cross-link, but not sulfated glycosaminoglycan. The marked changes in the tensile properties and collagen network components of articular cartilage with growth suggest that such parameters may be used to evaluate the degrees to which regenerated cartilage recapitulates normal development and growth. (C) 2003 Orthopaedic Research Society. Published by Elsevier Ltd. All rights reserved.