Intracellular transport of single-headed molecular motors KIF1A.
Intracellular transport of single-headed molecular motors KIF1A.
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DOI：
10.1103/physrevlett.95.118101
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发表时间：
2005-06
影响因子：
8.6
通讯作者：
K. Nishinari;Y. Okada;A. Schadschneider;D. Chowdhury
中科院分区：
文献类型：
--
作者：
K. Nishinari;Y. Okada;A. Schadschneider;D. Chowdhury
受单头驱动蛋白 KIF1A 实验的启发，我们开发了一种通过相互作用的分子马达进行细胞内运输的模型。它不仅明确地捕获了三磷酸腺苷水解的影响，还捕获了驱动单个电机的棘轮机制。我们的模型解释了在低密度极限下实验观察到的单分子特性，并预测了一个相图，该相图显示了水解和朗缪尔动力学对电机集体时空组织的影响。最后，我们在荧光标记的 KIF1A 体外实验中提供了域壁存在的实验证据。
Motivated by experiments on single-headed kinesin KIF1A, we develop a model of intracellular transport by interacting molecular motors. It captures explicitly not only the effects of adenosine triphosphate hydrolysis, but also the ratchet mechanism which drives individual motors. Our model accounts for the experimentally observed single-molecule properties in the low-density limit and also predicts a phase diagram that shows the influence of hydrolysis and Langmuir kinetics on the collective spatiotemporal organization of the motors. Finally, we provide experimental evidence for the existence of domain walls in our in vitro experiment with fluorescently labeled KIF1A.