COMBO: a defined freshwater culture medium for algae and zooplankton
COMBO: a defined freshwater culture medium for algae and zooplankton
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DOI：
10.1023/a:1003231628456
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发表时间：
1998-01-01
期刊：
影响因子：
2.6
通讯作者：
Herrera, L
中科院分区：
文献类型：
--
作者：
Kilham, SS;Kreeger, DA;Herrera, L
为了进行动物和食物生物之间相互作用的实验，有必要开发一种培养基，该培养基能够充分支持藻类和浮游动物的生长，而不需要改变培养基以适应藻类或动物。我们设计了一种名为COMBO的淡水介质，它支持藻类和浮游动物的良好生长。两种类型的藻类，Ankistrodesmus falcatus和Stephanodiscus hantzschii，饲养在COMBO和它们的生长速率没有显着不同的藻类生长在一个参考介质（WC）。其中一种藻类A. falcatus，然后喂给同样在COMBO中培养的枝藻Daphnia pulicaria，结果显示D.蚤类与在自然地表水中饲养的动物进行了比较。我们还确定了COMBO作为D.通过改变氮或磷浓度来影响Pulicaria，以评估新培养基是否可用于营养研究。降低COMBO的N、P含量对D.蚤状。其他研究人员也报告了COMBO中饲养的许多藻类和浮游动物的良好生长和繁殖。我们的研究结果表明，COMBO是一种有效的人工，确定的培养基能够支持强大的生长和繁殖的淡水藻类和浮游动物。
In order to conduct experiments on interactions between animals and food organisms, it is necessary to develop a medium that adequately supports the growth of both algae and zooplankton without the need to alter the medium to accommodate either the algae or the animals. We devised a freshwater medium, named COMBO, that supports excellent growth of both algae and zooplankton. Two types of algae, Ankistrodesmus falcatus and Stephanodiscus hantzschii, were reared in COMBO and their growth rates were not significantly different from those of algae grown in a reference medium (WC). One of these algae, A. falcatus, was then fed to a cladoceran, Daphnia pulicaria, which was also cultured in COMBO, and the resulting fecundities of D. pulicaria were compared to those of animals reared in natural surface water. We also determined whether the value of COMBO as a medium for D. pulicaria was affected by modifications in nitrogen or phosphorus concentration to evaluate whether the new medium will be useful in nutritional research. Lowering the N or P content of COMBO did not affect the reproductive performance of D. pulicaria. Other researchers have also reported excellent growth and reproduction by numerous algae and zooplankton reared in COMBO. Our results suggest that COMBO is an effective artificial, defined culture medium capable of supporting robust growth and reproduction of both freshwater algae and zooplankton.