Antigen capture enzyme-linked immunosorbent assay for specific detection of Reston Ebola virus nucleoprotein
Antigen capture enzyme-linked immunosorbent assay for specific detection of Reston Ebola virus nucleoprotein
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DOI：
10.1128/cdli.10.4.552-557.2003
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发表时间：
2003-07-01
期刊：
影响因子：
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通讯作者：
Morikawa, S
中科院分区：
文献类型：
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作者：
Ikegami, T;Niikura, M;Morikawa, S
抗原捕获酶联免疫吸附试验（ELISA）是快速检测埃博拉病毒最有效的方法之一。我们先前开发了使用单克隆抗体（MAb）3-3D的抗原捕获ELISA，其不仅与扎伊尔埃博拉病毒（EBO-Z）的核蛋白（NP）反应，而且与苏丹埃博拉病毒（EBO-S）和莱斯顿埃博拉病毒（EBO-R）的核蛋白反应。在这项研究中，我们开发了抗原捕获ELISA使用两种新的单克隆抗体，Res 2 - 6C 8和Res 2 - 1D 8，特异性的EBO-R的NP。Res 2 - 6C 8和Res 2 - 1D 8分别识别EBO-R NP的C-末端区域附近由4个和8个氨基酸残基组成的表位。使用这两种单克隆抗体的抗原捕获ELISA检测来自EBO-R感染的食蟹猴的组织中的EBO-R NP。使用Res 2 - 6C 8和Res 2 - 1D 8的抗原捕获ELISA可用于EBO-R感染的食蟹猴中NP的快速检测。
Antigen capture enzyme-linked immunosorbent assay (ELISA) is one of the most useful methods to detect Ebola virus rapidly. We previously developed an antigen capture ELISA using a monoclonal antibody (MAb), 3-3D, which reacted not only to the nucleoprotein (NP) of Zaire Ebola virus (EBO-Z) but also to the NPs of Sudan (EBO-S) and Reston Ebola (EBO-R) viruses. In this study, we developed antigen capture ELISAs using two novel MAbs, Res2-6C8 and Res2-1D8, specific to the NP of EBO-R. Res2-6C8 and Res2-1D8 recognized epitopes consisting of 4 and 8 amino acid residues, respectively, near the C-terminal region of the EBO-R NP. The antigen capture ELISAs using these two MAbs detected the EBO-R NP in the tissues from EBO-R-infected cynomolgus macaques. The antigen capture ELISAs using Res2-6C8 and Res2-1D8 are useful for the rapid detection of the NP in EBO-R-infected cynomolgus macaques.