Rodent brain imaging with SPECT/CT.
Rodent brain imaging with SPECT/CT.
复制标题
使用 SPECT/CT 进行啮齿动物脑部成像。
DOI：
10.1118/1.2710950
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发表时间：
2007
期刊：
影响因子：
3.8
通讯作者：
Franc,BenjaminL
中科院分区：
文献类型：
--
作者：
Seo,Youngho;Gao,Dong-Wei;Hasegawa,BruceH;Dae,MichaelW;Franc,BenjaminL
我们评价了使用专用于小动物成像的单光子发射计算机断层扫描（SPECT）系统（X‐SPECT™，Gamma Medica‐Ideas，Northridge，CA）对中风大鼠模型进行成像的方法。采用大脑中动脉闭塞（MCAO）技术建立了一种缺血性脑卒中动物模型，用于在体SPECT/CT成像。采用氯化三苯基四氮唑（TTC）染色法在体研究中证实脑缺血的存在，采用平行孔准直的exametazime动态平面成像法对MCAO后大鼠的脑血流进行在体放射性核素成像。随后立即使用1 mm针孔SPECT对相同动物进行体内放射性核素脑血流显像。进行相关计算机断层扫描成像以定位放射性药物摄取。使动物恢复，并在单独给予exametazime的情况下进行体内放射自显影。依西美他嗪的时间活性曲线显示，放射性药物摄取可维持超过。预计该活动在一个更长的时期内将保持相对稳定，尽管仅获得了2000年的数据。TTC染色可见脑梗死灶，SPECT在体显像显示脑血流障碍，其结果与活体放射自显影相一致。我们已经证明了成像区域的脑血流量不足的MCAO大鼠脑vivouing专用于小动物成像的针孔SPECT的能力。
We evaluated methods of imaging rat models of strokein vivousing a single photon emission computed tomography (SPECT) system dedicated to small animal imaging (X‐SPECT™, Gamma Medica‐Ideas, Northridge, CA). An animal model of ischemic stroke was developed forin vivoSPECT/CT imaging using the middle cerebral artery occlusion (MCAO) technique. The presence of cerebral ischemia was verified inex vivostudies using triphenyltetrazolium chloride (TTC) staining.In vivoradionuclide imaging of cerebral blood flow was performed in rats following MCAO using dynamic planar imaging of ‐exametazime with parallel hole collimation. This was followed immediately byin vivoradionuclide imaging of cerebral blood flow with ‐exametazime in the same animals using 1‐mm pinhole SPECT. Correlated computed tomography imaging was performed to localize radiopharmaceutical uptake. The animals were allowed to recover andex vivoautoradiography was performed with separate administration of ‐exametazime. Time activity curve of ‐exametazime showed that the radiopharmaceutical uptake could be maintained for over . The activity would be expected to be relatively stable for a much longer period, although the data were only obtained for . TTC staining revealed sizable infarcts by visual observation of inexistence of TTC stain in infracted tissues of MCAO rat brains.In vivoSPECT imaging showed cerebral blood flow deficit in the MCAO model, and thein vivoimaging result was confirmed withex vivoautoradiography. We have demonstrated a capability of imaging regions of cerebral blood flow deficit in MCAO rat brainsin vivousing a pinhole SPECT dedicated to small animal imaging.