Microsatellite analysis of fecal DNA for colorectal cancer detection
Microsatellite analysis of fecal DNA for colorectal cancer detection
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DOI：
10.1002/jso.10096
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发表时间：
2002-05-01
影响因子：
2.5
通讯作者：
Yoshioka, K
中科院分区：
文献类型：
--
作者：
Koshiji, M;Yonekura, Y;Yoshioka, K
背景和目标：方法：对30例散发性大肠癌和11例遗传性非息肉病性结肠癌（HNPCC）患者进行回顾性分析。我们使用7个微卫星位点（APC、p53、DCC、hMLH 1、D9 S 162、D9 S 17 1和IFNA）分析了从血液和手术标本（肿瘤和无肿瘤结肠粘膜）以及相应的口腔冲洗液和粪便标本中提取的匹配基因组DNA的杂合性丢失（洛）。为了减少粪便样本中正常结肠细胞DNA的污染，我们比较了三种不同的粪便基因组DNA提取方法。结果：15例正常人口腔冲洗液和粪便样本洛与血液和肿瘤组织LOH结果一致。所有HNPCC患者肿瘤DNA和相应粪便DNA中hMLH-1微卫星改变。p53-洛和D9 S171-洛在散发性和HNSCC组中的分布差异有统计学意义（P值分别为0.0031和0.0294）。2例非肿瘤患者D9 S162-洛缺失。对散发性大肠癌患者，p53和大肠腺瘤性息肉病（APC）检测的敏感性为96.7%（95%CI，83-100），特异性为100%。结论：口腔冲洗液和粪便标本的洛缺失分析可作为大肠癌的筛查工具。(C)2002 Wiley-Liss，Inc.
Background and Objectives: The advent of noninvasive methods of testing for colorectal cancer that have a high level of specificity and sensitivity is eagerly awaited.Methods: Thirty patients with sporadic colorcetal cancer and I I patients with hereditary nonpolyposis colon cancer (HNPCC) enrolled in this study. We analyzed the loss of heterozygosity (LOH) in matched genomic DNA extracted from blood and surgical specimens (tumor and tumor-free colonic mucosa), and the corresponding oral rinse and stool specimens using seven microsatellite loci (APC, p53, DCC, hMLH1, D9S 162, D9S 17 1, and IFNA). To reduce the normal colonocyte DNA contamination of the stool samples, we compared three different methods for fecal genomic DNA extraction. As normal controls, we analyzed the LOH using the oral rinse and stool samples from 15 individuals without cancer.Results: The LOH determined from the oral rinse and the stool samples matched those determined from the blood and the neoplastic tissue. All patients with HNPCC had microsatellite alterations at hMLH-1 in tumor DNA and corresponding fecal DNA. There were significant differences in the frequency of p53-LOH and D9S171-LOH between the group with sporadic disease and those with HNSCC (P = 0.0031 and 0.0294, respectively). Two cases with D9S162-LOH were detected in individuals without cancer. For the patients with sporadic disease, using p53 and adenomatous polyposis coli (APC), the sensitivity of the fecal DNA analysis Was 96.7% (95% Cl, 83-100) With a specificity of 100%.Conclusion: We demonstrate that LOH analysis using oral rinse and stool samples may be a suitable screening tool for colorectal cancer. (C) 2002 Wiley-Liss, Inc.