A Spanish case-control study in <5 year-old children reveals the lack of association between MLB and VA astrovirus and diarrhea
A Spanish case-control study in <5 year-old children reveals the lack of association between MLB and VA astrovirus and diarrhea
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DOI：
10.1038/s41598-020-58691-3
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发表时间：
2020-02-04
影响因子：
4.6
通讯作者：
Guix, Susana
中科院分区：
文献类型：
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作者：
Diem-Lan Vu;Sabria, Aurora;Guix, Susana
新型人类星状病毒 (HAstV) 于 10 年前被发现，与中枢神经系统感染的致命病例有关。它们在胃肠炎中的作用是有争议的，因为它们已在有症状和无症状的受试者中被发现。该研究的目的是在为期一年的胃肠炎病例对照研究中调查新型 HAstV，该研究包括西班牙的儿科人群。我们纳入了常规诊断筛查病毒呈阴性的胃肠炎患者的粪便样本，以及寻求常规医疗咨询的对照受试者的粪便样本。所有样本均通过实时 RT-PCR 检测来筛选新型 HAstV。还对对照组进行了轮状病毒、诺如病毒 GI、GII、沙波病毒、经典 HAstV 和腺病毒的额外筛查。总体而言，病例患者 (6.3%) 的 23/363 份粪便样本和对照患者 (4%) 的 8/199 份粪便样本对≥ 1 种新型 HAstV 呈阳性。 MLB1 占主导地位（64.5% 的阳性）。病例组观察到季节性（p = 0.015），但对照组没有观察到季节性（p = 0.95）。病例组和对照组之间新型 HAstV 的患病率没有观察到差异（OR 1.78，95% CI 0.68-5.45；p=0.30）。然而，对照组每毫升粪便悬浮液中的MLB基因组拷贝数显着高于病例组（p=0.008）。在我们的研究中，我们发现在研究人群中新型HAstV与胃肠炎之间缺乏关联，这可能表明儿童具有潜在的储存库作用，特别是考虑到在其中一些无症状群体中观察到的病毒载量较高。
Novel human astroviruses (HAstV) were discovered 10 years ago and have been associated with fatal cases of central nervous system infections. Their role in gastroenteritis is controversial, as they have been identified in symptomatic and asymptomatic subjects. The aim of the study was to investigate novel HAstV in a gastroenteritis case-control study including a pediatric population in Spain over a one-year period. We included stool samples from patients with gastroenteritis and negative results for viruses screened by routine diagnostics, and stool samples of control subjects who sought for a routine medical consultation. All samples were screened by real-time RT-PCR assays for novel HAstV. An additional screening for rotavirus, norovirus GI, GII, sapovirus, classic HAstV and adenovirus was also performed for the control group. Overall, 23/363 stool samples from case patients (6.3%) and 8/199 stool samples from control patients (4%) were positive for >= 1 novel HAstV. MLB1 was predominant (64.5% of positives). Seasonality was observed for the case group (p=0.015), but not the control group (p=0.95). No difference was observed in the prevalence of novel HAstV between the case and control groups (OR 1.78, 95% CI 0.68-5.45; p=0.30). Nevertheless, MLB genome copy numbers/ml of fecal suspension was significantly higher in the control group than in the case group (p=0.008). In our study, we identified a lack of association between novel HAstV and gastroenteritis in the studied population, which could indicate a potential role of reservoir for children, especially given the higher viral load observed in the asymptomatic group for some of them.