Effect of intracerebroventricular angiotensin II on body weight and food intake in adult rats
Effect of intracerebroventricular angiotensin II on body weight and food intake in adult rats
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DOI：
10.1152/ajpregu.00537.2003
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发表时间：
2004-08-01
影响因子：
2.8
通讯作者：
Potratz, KR
中科院分区：
文献类型：
--
作者：
Porter, JP;Potratz, KR
我们最近报道了在年轻大鼠脑室内注入ANG II减少了食物摄入并增加了能量消耗。本研究的目的是确定脑室内ANG II在成年大鼠中是否有类似的作用。我们还研究了这种效应的时间过程，认为在较早的时间点，下丘脑促肾上腺皮质激素释放激素（CRH）表达增加在厌食症中的潜在作用可以确定。最后，直接研究了ANG ii诱导的饮水对减少食物摄入量的贡献。大鼠通过渗透微型泵接受脑室盐水或ANG II。每天测量食物摄入量、水摄入量和体重。在开始注射后2、5或11天终止实验。angii(类似于32 ng.kg（-1）。min（-1）导致食物摄入量短暂减少，持续4-5天，尽管体重在整个实验中持续下降，这很可能是由于能量消耗增加，棕色脂肪组织中解偶联蛋白-1 mRNA表达增加。在第11天和第5天，CRH mRNA的表达降低。在第2天，即使体重下降，CRH表达也没有受到抑制。无论是否允许大鼠增加饮水量，食物摄入量和体重的减少都是相同的。这些数据表明，在成年大鼠的大脑中，ANG II以一种与水摄入无关的方式影响食物摄入和能量消耗。
We recently reported that intracerebroventricular infusions of ANG II decreased food intake and increased energy expenditure in young rats. The aim of the present study was to determine if intracerebroventricular ANG II has similar effects in adult rats. The time course of the effect was also investigated with the idea that at earlier time points, a potential role for increased hypothalamic expression of corticotropin-releasing hormone (CRH) in the anorexia could be established. Finally, the contribution of ANG II-induced water drinking to the decrease in food intake was directly investigated. Rats received intracerebroventricular saline or ANG II using osmotic minipumps. Food intake, water intake, and body weight were measured daily. Experiments were terminated 2, 5, or 11 days after the beginning of the infusions. ANG II (similar to32 ng.kg(-1).min(-1)) produced a transient decrease in food intake that lasted for 4-5 days although body weight continued to be decreased for the entire experiment most likely due to increased energy expenditure as evidenced by increased uncoupling protein-1 mRNA expression in brown adipose tissue. At 11 and 5 days, the expression of CRH mRNA was decreased. At 2 days, CRH expression was not suppressed even though body weight was decreased. The decrease in food intake and body weight was identical whether or not rats were allowed to increase water consumption. These data suggest that in adult rats ANG II acts within the brain to affect food intake and energy expenditure in a manner that is not related to water intake.