Amine Oxidases of Microorganisms:Part VII. An Improved Purification Procedure and Further Properties of Amine Oxidase of Aspergillus niger
Amine Oxidases of Microorganisms:Part VII. An Improved Purification Procedure and Further Properties of Amine Oxidase of Aspergillus niger
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微生物胺氧化酶：第七部分。
DOI：
10.1080/00021369.1969.10859530
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发表时间：
1969
期刊：
影响因子：
--
通讯作者：
Hideaki Yamada
中科院分区：
文献类型：
--
作者：
O. Adachi;Hideaki Yamada
本文介绍了一种从尼日尔曲霉菌丝体提取物中以较高收率制备结晶胺氧化酶的改进纯化方法。在以正丁胺为唯一氮源的培养基中，菌丝体中积累了胺氧化酶。用与发酵罐相连的pH调节器的碱槽加入正丁胺，使培养过程中培养基的pH值保持在5.0~5.5，盐酸胍对酶有抑制作用，当用含0.1M巯基乙醇的6 M盐酸胍透析时，酶发生解离。分子量为252，000的天然酶在透析过程中解离成三个亚基，每个亚基的分子量为85，000。该衍生物具有一定的生物活性，能够促进酵母菌的生长。荧光...
An improved purification procedure is described for preparation of crystalline amine oxidase in higher yields from the mycelial extract of Aspergillus niger. The amine oxidase was accumulated in mycelia of the fungus when it was grown in a medium containing n-butylamine as a sole nitrogen source. The n-butylamine was fed from the alkali tank of a pH stat connected with the jar fermenter, to maintain pH of the culture medium at 5.0~5.5 during the incubation.The amine oxidase was shown to be inhibited by guanidine hydrochloride and dissociation of the enzyme occurred when it was dialyzed against 6 m guanidine hydrochloride containing 0.1 m mercaptoethanol. The native enzyme of its molecular weight of 252,000 dissociated into three subunits of each molecular weight of 85,000 during the dialysis.Pyridoxal derivatives were detected in digest of the native enzyme by proteolysis followed by acid hydrolysis. The derivatives showed a biological activity to support the growth of Saccharomyces carlsbergensis. Fluore...