Hepatocarcinomas, cholangiocarcinomas, and hepatoblastomas produced by chemically transformed cultured rat liver epithelial cells. A light- and electron-microscopic analysis.
Hepatocarcinomas, cholangiocarcinomas, and hepatoblastomas produced by chemically transformed cultured rat liver epithelial cells. A light- and electron-microscopic analysis.
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发表时间：
1987-04
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通讯作者：
M. Tsao;J. Grisham
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作者：
M. Tsao;J. Grisham
本文研究了化学转化的大鼠肝上皮细胞系及其单细胞克隆亚群产生的100多个肿瘤的组织学和超微结构。观察到广泛的形态学表现，包括癌、肉瘤、“混合上皮-间质”肿瘤和未分化肿瘤。除表皮样癌和腺癌外，一些肿瘤在形态学上与肝细胞癌难以区分。“混合上皮间质”肿瘤再现了人类肝母细胞瘤的各种组织学特征。在许多情况下，上皮成分占据肿瘤的焦点区域，并且对“肉瘤”区域的透射电子显微镜研究揭示了梭形上皮肿瘤细胞，或者在大多数情况下，分散的上皮肿瘤细胞被许多成纤维细胞或肌成纤维细胞包围。在几个“肉瘤”超微结构检查中观察到类似的结果，这表明大多数混合瘤或肉瘤实际上是梭形细胞癌和/或具有明显宿主成纤维细胞反应的癌。然而，在少数由克隆来源的细胞株产生的混合瘤中，明确的癌细胞瘤与肿瘤性骨样或软骨样组织被证明。本研究的结果进行了讨论的背景下，目前的见解的细胞组成的肝脏和人类肝母细胞瘤的组织发生。
The histology and ultrastructure of more than 100 tumors produced by a chemically transformed rat liver epithelial cell line and its single-cell-derived clonal subpopulations were studied. Wide-ranging morphologic presentations were observed, including carcinomas, sarcomas, "mixed epithelial-mesenchymal" tumors, and undifferentiated tumors. In addition to epidermoid and adenocarcinomas, several tumors were morphologically indistinguishable from hepatocellular carcinomas. The "mixed epithelial-mesenchymal" tumors reproduced most of the various histologic features of human hepatoblastomas. In many instances, the epithelial component occupied focal areas of tumors, and transmission electron microscopic studies of the "sarcomatous" regions revealed either spindle-shaped epithelial tumor cells or, in most cases, scattered epithelial tumor cells surrounded by numerous fibroblasts or myofibroblasts. Similar findings were observed in several "sarcomas" examined ultrastructurally, which suggests that most of the mixed tumors or sarcomas actually were spindle cell carcinomas and/or carcinomas with marked host fibroblastic reaction. However, in a few mixed tumors produced by clonally derived cell strains, unequivocal carcinosarcomas with neoplastic osteoid or chondroid tissue were demonstrated. The findings of this study are discussed in the context of current insights on the cellular composition of the liver and on the histogenesis of human hepatoblastoma.