EFFECTS OF NITRATE AND PLANT DEVELOPMENT ON THE ABUNDANCE OF NITROGENOUS SOLUTES IN ROOT-BLEEDING AND VACUUM-EXTRACTED EXUDATES OF SOYBEAN
EFFECTS OF NITRATE AND PLANT DEVELOPMENT ON THE ABUNDANCE OF NITROGENOUS SOLUTES IN ROOT-BLEEDING AND VACUUM-EXTRACTED EXUDATES OF SOYBEAN
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DOI：
10.2135/cropsci1984.0011183x002400010041x
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发表时间：
1984-01-01
期刊：
影响因子：
2.3
通讯作者：
HERRIDGE, DF
中科院分区：
文献类型：
--
作者：
HERRIDGE, DF
在所报道的实验中，酰脲的相对含量[（酰脲-N/酰脲-N+硝酸根N + α-N）]为0.01%。氨基-N）×100]的木质素分泌物和相对共生依赖性的大豆接种根瘤菌，比较了温室生长的植物提供了不同浓度的硝酸盐范围从0-8.0毫米。木质素分泌物取样从植物的真空提取分离的芽或根出血分泌物。共生体的依赖，每个NO3-处理计算的基础上，总植物N和时间积分速率的固氮酶（C2 H2还原）活动。分析2种渗出物的所有样品的酰脲、NO3-和α- NH 2-N和样品从R2植物的个别氨基化合物显示，只有轻微的差异，在这些化合物的组合物的2个渗出物。相对于伤根渗出物，氨基酸和酰胺类对真空提取的渗出物中总氮的贡献稍大，酰脲类和NO3-对总氮的贡献稍小。光合氨基酸丙氨酸和丝氨酸在茎分泌物中的含量较高。供NO3-和株龄对两种分泌物酰脲相对含量的影响相似。这些数据相结合的NO3-供应对N2（C2 H2）固定的影响的数据来描述标准曲线之间的关系的共生依赖的植物和酰脲的相对含量为每个8个生长阶段。
In the experiments reported, the relative contents of ureides [(ureide-N/ureide-N + nitrate N + .alpha.-amino-N) .times. 100] of xylary exudates and the relative symbiotic dependence of nodulated soybean [inoculated with Rhizobium japonicum], were compared using glasshouse-grown plants supplied with various concentrations of nitrate ranging from 0-8.0 mM. Xylary exudates were sampled from the plants by vacuum extraction of detached shoots or as root-bleeding exudate. Symbiotic dependence for each of the NO3- treatments was calculated on the basis of total-plant N and time-integrated rates of nitrogenase (C2H2 reduction) activities. Analyses of all samples of the 2 exudates for ureides, NO3- and .alpha.-NH2-N and of samples from R2 plants of individual amino compounds revealed only minor differences between the 2 exudates in the composition of these compounds. Amino acids and amides contributed slightly more and ureides and NO3- slightly less to total N of vacuum-extracted exudate relative to root-bleeding exudate. The photosynthetic amino acids, alanine and serine, were more evident in stem exudate. Effects of NO3- supply and plant age on the relative contents of ureides were similar for both exudates. These data were combined with the data on the effects of NO3- supply on N2(C2H2) fixation to describe standard curve relationships between the symbiotic dependence of the plants and the relative contents of ureides for each of 8 phases of growth.