Characterization of rehydrated gelatin gels.
Characterization of rehydrated gelatin gels.
复制标题
再水化明胶凝胶的表征。
DOI：
10.1111/j.1525-1594.1991.tb00756.x
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发表时间：
1991
影响因子：
2.4
通讯作者：
Nosé,Y
中科院分区：
文献类型：
--
作者：
Emoto,H;Kambic,H;Chen,JF;Nosé,Y
5%戊二醛交联凝胶作为心脏假体涂层显示出优异的血液相容性。开发了一种使用专有脱水程序和环氧乙烷（EO）灭菌生产薄脱水涂层的方法。比较了再水化物与湿（原始）凝胶的溶胀表面。在盐水再水化30 min内，毛面上的干燥30-50 μm薄膜变得光滑、均匀，与原始明胶凝胶相当。再水化后的机械测试结果显示应变值保持不变（39.3 ± 10.0至40.0 ± 7.8%），但应力增加（2.79 ± 1.21至4.22 ± 1.60达因/cm 2; p < 0.01）。原始和再水化凝胶的接触角数据报告的± C值为26.1和30.4达因/cm。使用库仑滴定计，干燥后原始凝胶的测量水含量从85%降低到3.4%。干燥和盐水再水化的凝胶具有73.2%的水分含量。凝胶收缩8.8%的原始长度后，再水化，然而，所有泵涂层的厚度保持稳定。与原始凝胶相比，用牛富血小板血浆孵育2小时后，血小板反应性或形态无差异。光学显微镜和扫描电子显微镜（SEM）显示，在泵耐久性测试3周和5周后，没有凝胶破裂或表面缺陷的证据。脱水过程消除了这种水凝胶的湿储存和无菌问题，并为各种血液接触基材提供了稳定的膜涂层。
Five percent glutaraldehyde cross–linked gels have shown excellent blood compatibility as coatings for cardiac prostheses. A method was developed for producing thin dehydrated coatings using a proprietary dehydration procedure and ethylene oxide (EO) sterilization. The swollen surfaces of rehydrates versus wet (original) gels were compared. Within 30 min of saline rehydration, dry 30–50–μm films on textured surfaces became smooth, uniform, and comparable to original gelatin gels. Mechanical test results after rehydration showed values for strain remain unchanged (39.3 + 10.0 to 40.0 + 7.8%), but stress increased (2.79 + 1.21 to 4.22 + 1.60 dyne/cm2; p < 0.01). The contact angle data reported ±Cvalues of 26.1 and 30.4 dyne/cm for original and rehydrated gels. Using a coulometric titrimeter, the measured water content of original gels was reduced from 85 to 3.4% after drying. Dried and saline rehydrated gels had a 73.2% moisture content. Gels shrank 8.8% of their original length after rehydration; however, the thickness of all pump coatings remained stable. Two–hour incubations with bovine platelet rich plasma showed no differences in platelet reactivity or morphology when compared to original gels. Light microscopy and scanning electron microscopy (SEM) showed no evidence of gel cracking or surface defects after pump endurance testing for 3 and 5 weeks. The process of dehydration eliminates the wet storage and sterility problems of such hydrogels and provides a stable film coating for a variety of blood–contacting substrates.