AtSTP3, a green leaf-specific, low affinity monosaccharide-H+ symporter of Arabidopsis thaliana
AtSTP3, a green leaf-specific, low affinity monosaccharide-H+ symporter of Arabidopsis thaliana
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DOI：
10.1046/j.1365-3040.2000.00538.x
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发表时间：
2000-02-01
影响因子：
7.3
通讯作者：
Sauer, N
中科院分区：
文献类型：
--
作者：
Büttner, M;Truernit, E;Sauer, N
本文介绍了拟南芥AtSTP 3基因的表达分析，其编码蛋白作为一种新的单糖转运蛋白的功能特性，并介绍了AtSTP基因家族。AtSTP 3蛋白（糖转运蛋白3）的动力学特性进行了研究，在一个己糖转运缺陷突变体裂殖酵母粟酒裂殖酵母。AtSTP 3代表一种新的单糖转运蛋白，由514个氨基酸组成，计算分子量为55.9 kDa。在酵母中的动力学分析表明，AtSTP 3是一个低亲和力，能量依赖的H+同向转运体，与一个K-m为2 mM的D-葡萄糖。RNA酶保护分析表明，AtSTP 3在拟南芥的叶片和花组织中表达。AtSTP 3基因的这种表达模式在AtSTP 3启动子-β-葡萄糖醛酸酶（GUS）植物中得到证实，所述植物在绿色叶（例如子叶、莲座叶和茎叶以及萼片）中显示AtSTP 3驱动的GUS活性。损伤诱导转基因植株中GUS活性的增加和拟南芥野生型植株中AtSTP 3 mRNA水平的增加。聚合酶链反应分析与简并引物确定了额外的新的AtSTP基因，并显示AtSTP 3是一个大家族的成员，至少有14个同源基因编码推定的单糖-H+同向转运体（AtSTPs）。
This paper describes the expression analyses of the AtSTP3 gene of Arabidopsis thaliana, the functional characterization of the encoded protein as a new monosaccharide transporter, and introduces the AtSTP gene family. The kinetic properties of the AtSTP3 protein (for sugar transport protein 3) were studied in a hexose transport deficient mutant of Schizosaccharomyces pombe. AtSTP3 represents a new monosaccharide transporter that is composed of 514 amino acids and has a calculated molecular mass of 55.9 kDa. Kinetic analyses in yeast showed that AtSTP3 is a low affinity, energy-dependent H+ symporter with a K-m for D-glucose of 2 mM. RNase protection analyses revealed that AtSTP3 is expressed in leaves and floral tissue of Arabidopsis. This expression pattern of the AtSTP3 gene was confirmed in AtSTP3 promoter-beta-glucuronidase (GUS) plants showing AtSTP3-driven GUS activity in green leaves, such as cotelydons, rosette and stalk leaves and sepals. Wounding caused an induction of GUS activity in the transgenic plants and an increase of AtSTP3 mRNA levels in Arabidopsis wild-type plants. Polymerase chain reaction analyses with degenerate primers identified additional new AtSTP genes and revealed that AtSTP3 is the member of a large family of at least 14 homologous genes coding for putative monosaccharide-H+ symporters (AtSTPs).