MicroRNA profiling of adrenocortical tumors reveals miR-483 as a marker of malignancy.
MicroRNA profiling of adrenocortical tumors reveals miR-483 as a marker of malignancy.
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DOI：
10.1002/cncr.25724
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发表时间：
2011-04-15
期刊：
影响因子：
6.2
通讯作者：
Kebebew, Electron
中科院分区：
文献类型：
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作者：
Patterson, Erin E;Holloway, Alisha K;Weng, Julie;Fojo, Tito;Kebebew, Electron
我们有兴趣确定有助于诊断肾上腺皮质癌（ACC）的分子标记。本研究的目的是鉴定与良性肿瘤相比在恶性肾上腺皮质肿瘤中差异表达的 microRNA（miRNA 或 miR），并评估它们作为诊断预测因子的潜力。使用肾上腺皮质肿瘤的微阵列分析来鉴定差异表达的 miRNA，并通过定量实时 RT-PCR 进行验证。良性和原发性恶性肾上腺皮质肿瘤的微阵列分析揭示了这些组织学组之间的许多显着差异。对这些差异表达的 miRNA 的子集进行定向定量 RT-PCR 分析，我们确定与良性肿瘤相比，恶性肿瘤中 miR -100、-125b 和 -195 显着下调，miR-483-5p 显着上调。此外，我们的工作表明 miR-483-5p 表达可以准确地将肿瘤分类为良性或恶性。我们发现了四种在肾上腺皮质癌中失调的 miRNA。其中一种 miR-483-5p 的高表达似乎是肾上腺皮质恶性肿瘤的一个定义特征，可用于准确区分良性和恶性肾上腺皮质肿瘤。
We are interested in identifying molecular markers that can aid in the diagnosis of adrenocortical carcinoma (ACC). The aim of this study was to identify microRNAs (miRNAs or miRs) that are differentially expressed in malignant adrenocortical tumors as compared to benign tumors and assess their potential as diagnostic predictors. Differentially expressed miRNAs were identified using microarray profiling of adrenocortical tumors and validated by quantitative real-time RT-PCR. Microarray profiling in benign and primary malignant adrenocortical tumors revealed a number of significant differences between these histological groups. Using directed quantitative RT-PCR analysis on a subset of these differentially expressed miRNAs, we determined that miRs −100, −125b, and −195 were significantly downregulated and miR-483-5p was significantly upregulated in malignant as compared to benign tumors. Furthermore, our work shows that miR-483-5p expression can accurately categorize tumors as benign or malignant. We identified four miRNAs that are dysregulated in adrenocortical carcinoma. The high expression of one of these, miR-483-5p, appears to be a defining characteristic of adrenocortical malignancies and can be used to accurately distinguish between benign and malignant adrenocortical tumors.