Bishydrazide Glycoconjugates for Lectin Recognition and Capture of Bacterial Pathogens
Bishydrazide Glycoconjugates for Lectin Recognition and Capture of Bacterial Pathogens
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DOI：
10.1021/bc100288c
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发表时间：
2010-11-01
影响因子：
4.7
通讯作者：
Wei, Alexander
中科院分区：
文献类型：
--
作者：
Adak, Avijit Kumar;Leonov, Alexei P.;Wei, Alexander
双肼是用于将聚糖连接至底物以用于凝集素结合和病原体检测方案的通用接头。羧甲基化六（乙二醇）（4）的α，ω-双肼可以在一端与未保护的寡糖缀合，然后附着在载体蛋白上，栓系在活化的羧基封端表面上，或用光敏交联剂官能化以进行平版印刷图案化。双酰肼4的糖缀合物也可以通过在温和条件下用CS2处理转化为二硫代氨基甲酸酯（DTC），用于附着到金基底上。固定化的聚糖作为细胞表面凝集素的识别元件，并通过其吸附到微图案化基底上来检测和捕获细菌病原体，例如铜绿假单胞菌。使用最近引入的基于光学模式识别的方法，证明检测限为10（3）cfu/mL。
Bishydrazides are versatile linkers for attaching glycans to substrates for lectin binding and pathogen detection schemes. The alpha,omega-bishydrazides of carboxymethylated hexa(ethylene glycol) (4) can be conjugated at one end to unprotected oligosaccharides, then attached onto carrier proteins, tethered onto activated carboxyl-terminated surfaces, or functionalized with a photoactive cross-linking agent for lithographic patterning. Glycoconjugates of bishydrazide 4 can also be converted into dithiocarbamates (DTCs) by treatment with CS2 under mild conditions, for attachment onto gold substrates. The immobilized glycans serve as recognition elements for cell-surface lectins and enable the detection and capture of bacterial pathogens such as Pseudomonas aeruginosa by their adsorption onto micropatterned substrates. A detection limit of 10(3) cfu/mL is demonstrated, using a recently introduced method based on optical pattern recognition.