Thirteen week toxicity study of dietary L-tryptophan in rats with a recovery period of 5 weeks.
Thirteen week toxicity study of dietary L-tryptophan in rats with a recovery period of 5 weeks.
复制标题
膳食 L-色氨酸对大鼠的十三周毒性研究，恢复期为 5 周。
DOI：
10.1002/jat.3562
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发表时间：
2018
期刊：
影响因子：
3.3
通讯作者：
and Sakai R.
中科院分区：
文献类型：
--
作者：
Shibui Y;Matsumoto H;Masuzawa Y;Ohishi T;Fukuwatari T;Shibata K;and Sakai R.
尽管l-色氨酸在营养上很重要，并广泛用于医学应用，但其口服给药的毒性数据有限。本研究的目的是评价Sprague-Dawley大鼠连续13周给予含添加的dl-色氨酸的实验饲料（剂量为0（基础饲料）、1.25%、2.5%和5.0%）的潜在毒性。对照组大鼠和喂食添加全色氨酸的大鼠之间的临床体征、眼科学、尿分析、血液学、尸检、器官重量和组织病理学无毒理学变化。在整个给药期间，2.5%组雄性动物和5.0%组两种性别动物的体重增量和摄食量均显著降低。给药期结束时，观察到5.0%组雄性动物的饮水量降低和5.0%组雌性动物的血清葡萄糖降低。认为上述变化具有毒理学显著性;然而，在5周恢复期后未观察到这些变化，表明具有可逆性。因此，本研究中L-色氨酸的无明显不良作用水平为1.25%（雄性）和2.5%（雌性）（L-色氨酸的平均摄入量：779 mg kg-1体重/天[雄性]和1765 mg kg-1体重/天[雌性]）。由于本研究中使用的基础饲料含有0.27%的蛋白质色氨酸，因此雄性和雌性的总色氨酸的无明显不良作用水平分别为1.52%和2.77%（总色氨酸的平均摄入量：948 mg kg-1体重/天（雄性）和1956 mg kg-1体重/天（雌性））。我们得出结论，l-色氨酸在人类使用方面具有低毒性特征。
Althoughl‐tryptophan is nutritionally important and widely used in medical applications, toxicity data for its oral administration are limited. The purpose of this study was to evaluate the potential toxicity of an experimental diet containing addedl‐tryptophan at doses of 0 (basal diet), 1.25%, 2.5% and 5.0% when administered to Sprague–Dawley rats for 13 weeks. There were no toxicological changes in clinical signs, ophthalmology, urinalysis, hematology, necropsy, organ weight and histopathology between control rats and those fed additionall‐tryptophan. Body weight gain and food consumption significantly decreased throughout the administration period in males in the 2.5% group and in both sexes in the 5.0% group. At the end of the dosing period, decreases in water intake in males in the 5.0% group and in serum glucose in females in the 5.0% group were observed. The changes described above were considered toxicologically significant; however, they were not observed after a 5 week recovery period, suggesting reversibility. Consequently, the no‐observed‐adverse‐effect level ofl‐tryptophan in the present study was 1.25% for males and 2.5% for females (mean intake ofl‐tryptophan: 779 mg kg–1body weight day–1[males] and 1765 mg kg–1body weight day–1[females]). As the basal diet used in this study contained 0.27% of proteinaceousl‐tryptophan, the no‐observed‐adverse‐effect level of overalll‐tryptophan was 1.52% for males and 2.77% for females (mean intake of overalll‐tryptophan: 948 mg kg–1body weight day–1(males) and 1956 mg kg–1body weight day–1(females)). We conclude thatl‐tryptophan has a low toxicity profile in terms of human use.