A linkage map of endogenous murine leukemia proviruses.
A linkage map of endogenous murine leukemia proviruses.
复制标题
内源性小鼠白血病原病毒的连锁图。
DOI：
10.1093/genetics/124.2.221
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发表时间：
1990
期刊：
影响因子：
3.3
通讯作者：
Coffin,JM
中科院分区：
文献类型：
--
作者：
Frankel,WN;Stoye,JP;Taylor,BA;Coffin,JM
在几组重组近交系小鼠中，通过原病毒-细胞DNA连接片段分离鉴定了30个属于鼠白血病病毒（MLV）的改良多变型（Mpmv）类的内源性原病毒。26个Mpmv基因座被映射到染色体区域匹配前病毒株分布模式，以前分配的基因。像其他内源性nonecotropic MLV，Mpmv基因座存在于几个染色体上的所有菌株进行了检查。我们汇集了重组近交系连锁数据从110个MLV位点和选择的标记基因，以构建染色体连锁图谱。每一个小鼠染色体被发现窝藏至少一个前病毒插入，和几个区域包含多个整合。然而，110个映射的前病毒的总体分布没有显著偏离随机分布。由于它们在小鼠近交系中的多态性，以及仅使用三种杂交探针就能对每个品系多达57个前病毒进行评分的能力，在常见小鼠品系中定位的非亲嗜性MLV提供了优于旧方法的显著优势（例如，生物化学或单个限制性片段多态性）作为遗传标记。这些内源性插入元件也应该是有用的，用于评估菌株纯度，并研究常见和不常见的近交系的相关性。
Thirty endogenous proviruses belonging to the modified polytropic (Mpmv) class of murine leukemia virus (MLV) were identified by proviral-cellular DNA junction fragment segregation in several sets of recombinant inbred mice. Twenty-six Mpmv loci were mapped to chromosomal regions by matching proviral strain distribution patterns to those of previously assigned genes. Like other endogenous nonecotropic MLVs, Mpmv loci were present on several chromosomes in all strains examined. We pooled recombinant inbred strain linkage data from 110 MLV loci and selected marker genes in order to construct a chromosomal linkage map. Every mouse chromosome was found to harbor at least one proviral insertion, and several regions contained multiple integrations. However, the overall distribution of the 110 mapped proviruses did not deviate significantly from a random distribution. Because of their polymorphism in inbred strains of mice, and the ability to score as many as 57 proviruses per strain using only three hybridization probes, the nonecotropic MLVs mapped in common strains of mice offer a significant advantage over older methods (e.g., biochemical or individual restriction fragment polymorphisms) as genetic markers. These endogenous insertion elements should also be useful for assessing strain purity, and for studying the relatedness of common and not-so-common inbred strains.